Strain Information | |
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Image | |
BRC No. | RBRC00782 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Gipr<tm1Yse>/Rbrc |
Former Common name | GIPR Knockout mice |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrSlc |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Yutaka Seino, Kyoto University in 1999. A neomycin cassette was inserted to replace exons 4 and 5 of GIPR gene. The mutant mice were backcrossed to C57BL/6. |
Strain description | B6.129P2-Gipr<tm1Yse>. Gastric inhibitory polypeptide (GIP) is a gastrointestinal peptide hormone synthesized by K cells in the duodenum and small intestine, GIP plays a role in gastric secretory inhibition, gastric mobility, and insulin release by binding to its specific receptor, the GIP receptor (GIPR). Homozygous mutant mice for Gipr<tm1Yse> targeted mutation are fertile and viable, but exhibit hyperglycemia after high-fat diet ingestion. This strain is useful for elucidating of the signaling mechanism of GIP from pancreatic beta cell to intestine. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)Homozygous mice are fertile and viable. |
References | Proc. Natl. Acad. Sci. USA, 96, 14843-14847 (1999). 10611300 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse GIP receptor genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter) |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Miyawaki K, et al. Proc Natl Acad Sci USA, 96, 14843-14847 (1999). |
Depositor | Yutaka Seino (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Genetic Background |
BRC mice in Publications |
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Iida A, Seino Y, Fukami A, Maekawa R, Yabe D, Shimizu S, Kinoshita K, Takagi Y, Izumoto T, Ogata H, Ishikawa K, Ozaki N, Tsunekawa S, Hamada Y, Oiso Y, Arima H, Hayashi Y. Endogenous GIP ameliorates impairment of insulin secretion in proglucagon-deficient mice under moderate beta cell damage induced by streptozotocin. Diabetologia 59(7) 1533-1541(2016) 27053237 |