Strain Information | |
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Image | |
BRC No. | RBRC00892 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6-Tg(CAG-EGFP,-Aicda)20Hon/HonRbrc |
Former Common name | AID-conditional Transgenic mouse line 20 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrSlc |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Drs. Tasuku Honjo and Ilmi Okazaki at Kyoto University in 2002. |
Strain description | These transgenic mice constitutively express GFP under control of CAG promoter. When crossed with a Cre recombinase-expressing strain, GFP expression is replaced with mouse AID expression in tissue expressing Cre. AID, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation, playing a central role in acquisition for B cell of antibody gene variety. |
Colony maintenance | Backcross to C57BL/6 (Hemizygote x C57BL/6CrSlc) |
References | Proc. Natl. Acad. Sci. USA, 103, 2752-2757 (2006). 16477013 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, Jellyfish GFP cDNA, mouse Aicda cDNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System Immunology and Inflammation Research |
Specific Term and Conditions | 1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Proc. Natl. Acad. Sci. USA, 103, 2752-2757 (2006). 3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE. |
Depositor | Tasuku Honjo (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |