Strain Information | |
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Image | |
BRC No. | RBRC00983 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-Gata2<tm1Mym>/Rbrc |
Former Common name | GATA-2 2nd Exon GFP knock in |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6J |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Drs. Naoko Minegishi and Masayuki Yamamoto, Center for Tukuba Advanced Research Alliance, University of Tsukuba in 2003. GFP was inserted in exon 2 of the Gata2 gene (Blood, 2003). |
Strain description | B6;129P2-Gata2<tm1Mym>. GFP knock-in mice in the Gata2 locus, replacing the endogenous Gata2 coding sequences in the second exon. GFP expression was observed in the early hematopoietic tissues. Homozygous mutat mice are embryonic lethal (E11.0). |
Colony maintenance | Heterozygote x Wild-type [C57BL/6J]Homozygous mutat mice are embryonic lethal (E11.0). |
References | Blood, 102, 896-905 (2003). 12689939 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)6 |
Ordering Information | |
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Donor DNA | Jellyfish GFP cDNA, mouse GATA-2 genomic DNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in articles when the users publish their data using these mice for 2 year after provided. Users should contact the depositor in the case of application for any patents with the results from these mice. |
Depositor | Masayuki Yamamoto (University of Tsukuba) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |