Strain Information | |
---|---|
Image | |
BRC No. | RBRC01661 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | ICR.Cg-Grm1<tm1(GFP)>/Rbrc |
Former Common name | GluR1<neo> |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Drs. Tatsumi Hirata and Yumiko Saga in National Institute of Genetics at 2005. PGK-neo flanked by loxP sites linked to a EGFP gene was inserted into the second exon of Grm1 gene of TT2 ES cells. The expression of the EGFP was not detected. |
Strain description | Grm1 (glutamate receptor, metabotropic 1) is a member of a large family of G-protein-coupled glutamate receptor that is a key form normal synapse formation, synaptic plasticity, and motor control in the cerebellum. Mice deficient in mGluR1 have severe motor coordination. Homozygous mutant mice show severe motor coordination in 10-14 days old. |
Colony maintenance | Cross to ICR (Heterozygote x Slc:ICR) |
References |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)10 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
---|---|
Donor DNA | bacterial neomycin, jellyfish enhanced green fluorescent protein gene, phage P1 loxP, mouse mGluR1 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter) |
Research application | |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Tastumi Hirata (National Institute of Genetics) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |