Strain Data Sheet
RBRC03666
Strain Information | |
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| Image | |
| BRC No. | RBRC03666 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | STOCK Arx<tm1Kki> Gad1<tm1.1Tama>/Rbrc |
| Former Common name | Arx-6 KO/GAD67 GFPdNeo KI |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences. |
| Strain description | Arx gene knockout and GAD67-GFP (Gad1-GFPdNeo) double mutant mice. Arx: A lacZ-neo cassette was inserted into the exon 2 of Arx gene. Hemizygous (-/Y) mutant males die within 12 hrs after birth. GAD67: An EGFP was inserted at the ATG codon in exon 1 of Gad1. Homozygous mutant mice are rethal. A loxP flanked neo was removed by crossing with cre mice. |
| Colony maintenance | Arx: Heterozygote (female) x Wild-type (male); Gad1: Heterozygote x Wild-type [or Crossing to C57BL/6JJcl] |
| References | Nat Genet. 2002 32(3):359-69. 12379852 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)2EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)X Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)X Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)Xmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal |
Ordering Information | |
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| Donor DNA | STOP sequence, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), beta-galactosidase (E. coli), PolyA signal (SV40), mouse phosphoglycerate kinase promoter (PGK promoter), Neomycin resistance gene (E. coli), ARX genomic DNA (mouse), GFP cDNA (Jellyfish), phage P1 loxP site, Gad1 genomic DNA (mouse) |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization. The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation. The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution. |
| Depositor | Kunio Kitamura (Mitsubishi Kasei Institute of Life Sciences) |
| Strain Status |  Frozen embryos |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |