Strain Information | |
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Image | |
BRC No. | RBRC04021 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Dnase2a<tm1Osa> Ifnar1<tm1Agt> |
Former Common name | DNaseII / IFN-IR double knock out |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | C57BL/6 |
Appearance | |
Strain development | Developed by Kohki Kawane and Shigekazu Nagata, Graduate School of Medicine, Kyoto University in 2004. C57BL/6 background (over N8 generations). |
Strain description | DNaseII/IFN-IR double knockout mice. Double homozygous mutant mice develop polyarthritis as age. |
Colony maintenance | Dnase2a: Heterozygote x Wild-type; Ifnar1: Homozygote x Homozygote |
References | Nat. Immunol., 6, 49-56 (2005). 15568025 Science, 264, 1918-1921 (1994). 8009221 Nature, 443, 998-1002 (2006). 17066036 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)16herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP sequence, Mouse Pgk1 genomic DNA, E. coli Neomycin resistance gene, mouse Dnase2a genomic DNA, Herpes simplex virus Thymidine kinase promoter (HSV-tk promoter), Polyoma virus genomic DNA, mouse Ifnar1 genomic DNA [update March 10, 2014] |
Research application | Cre/loxP system Immunology and Inflammation Research Mouse Models for Human Disease |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 443, 998-1002 (2006). Nat. Immunol., 6, 49-56 (2005).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shigekazu Nagata (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Mouse of the Month Aug 2012 Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Yoshikawa R, Sakabe S, Urata S, Yasuda J. Species-Specific Pathogenicity of Severe Fever with Thrombocytopenia Syndrome Virus Is Determined by Anti-STAT2 Activity of NSs. J Virol 93(10) (2019) 30814285 |