Strain Data Sheet

RBRC04133

Strain Information

Image
BRC No.RBRC04133
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Grb2<Gt(LTOPG)22Osb>
Former Common nameLTOPG-22:Grb2
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Masato Ikawa, Research Institute for Microbial Diseases, Osaka University in 2009.
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Grb2growth factor receptor bound protein 211Grb2<Gt(LTOPG)22Osb>gene trap 22, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)11mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)11WPRE

Ordering Information

Donor DNALentiviral Vector LT-OPG(LTR; Long terminal repeats, cPPT; Central polypurine tract, Psi (Ψ), RRE), mouse phosphoglycerate kinase promoter (PGK promoter), Jellyfish GFP cDNA, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), 3ic, SD (splice donor sequence))
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data