Strain Data Sheet

RBRC04199

Strain Information

Image
BRC No.RBRC04199
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cxcl12<tm1Tng>
Former Common nameCXCL12 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Takashi Nagasawa, Resrarch institute, Osaka Medical Center for Maternal and Child Health.
Strain description
Colony maintenance
ReferencesNature, 382, 635-638 (1996). 8757135

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cxcl12chemokine (C-X-C motif) ligand 126Cxcl12<tm1Tng>targeted mutation 1, Takashi Nagasawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAHSV thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, HSV thymidine kinase polyA signal, Mouse Cxcl12 genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
DepositorTakashi Nagasawa (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Greenbaum A, Hsu YM, Day RB, Schuettpelz LG, Christopher MJ, Borgerding JN, Nagasawa T, Link DC.
CXCL12 in early mesenchymal progenitors is required for haematopoietic stem-cell maintenance.
Nature 495(7440) 227-30(2013) 23434756