Strain Information | |
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Image | |
BRC No. | RBRC04420 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129(SJL)-Tcirg1<tm1.1Ywd> |
Former Common name | a3-GFP, Tcirg1<floxGFP> |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University in 2008. This allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon. |
Strain description | This allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon. The modified gene encodes a fusion protein in which the C-terminal of TCIRG1 is joined to the N-terminal of EGFP by the linker sequence AALELVDPPGSIAT. EGFP fluorescence and the immunofluorescence signal for the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, are colocalized in the late endocytic compartments of mutant peritoneal macrophages. Cre recombinase excision of the loxP-flanked DNA segment generates a null allele. |
Colony maintenance | |
References | J. Cell Sci., 122, 2504-2513 (2009). 19549681 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Tcirg1T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A319Tcirg1<tm1.1Ywd>targeted mutation 1.1, Yoh Wada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site19Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)19 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP |
Ordering Information | |
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Donor DNA | Phage phage P1 loxP sites, Yeast flipase recombination target site, Jellyfish GFP cDNA, Mouse Tcirg1 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Yoh Wada (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |