Strain Information | |
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Image | |
BRC No. | RBRC05431 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Ano5<tm1Itak> |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Hiroshi Inoue, Satoshi Tsutsumi, Mitsuo Itakura, Institute for Genome Research, The University of Tokushima and Kuniko Mizuta, Hiroshima University Graduate School of Biomedical Sciences. A neomycin selection cassette was transferred into C57BL/6 derived ES cells to replace exon 1 of Ano5 gene. |
Strain description | Ano5, anoctamin 5 (Gdd1) knockout mice. The first exon of Ano5 gene was replaced with a neomycin selection cassette. Homozygous mutant mice show no obvious abnormality. |
Colony maintenance | |
References | Biochem. Biophys. Res. Commun., 357, 126-132 (2007). 17418107 Am. J. Hum. Genet., 86, 213-221 (2010). 20096397 Biochem. Biophys. Res. Commun., 331, 1099-1106 (2005). 15882990 Am. J. Hum. Genet., 74, 1255-1261 (2004). 15124103 J. Bone Miner. Res., 18, 413-418 (2003). 12619924 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli. neo, mouse GDD1 (ANO5) genomic DNA |
Research application | Mouse Models for Human Disease |
Specific Term and Conditions | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Mitsuo Itakura (Tokushima University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Xu J, El Refaey M, Xu L, Zhao L, Gao Y, Floyd K, Karaze T, Janssen PM, Han R. Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy. Skelet Muscle 5 43(2015) 26693275 |