Strain Information | |
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Image | |
BRC No. | RBRC05659 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ly75<tm1.1Ksat> |
Former Common name | Cd205<dtr>, B6- Ly75/DT-GFP KI |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2009. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. The mutant mice were backcross to C57BL/6J. |
Strain description | IRES-DTR-EGFP cassette were inserted in to 3’ untranslated region of CD205 (Ly75) gene. In mice with diphtheria toxin (DT) induced specific ablation of cd205 positive conventional dendritic cells (cDCs). |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)] |
References | Proc. Natl. Acad. Sci. USA, 109, 11288-11293 (2012). 22736794 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)2EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBEGFheparin-binding EGF-like growth factor (human)2HBEGF Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRES2internal ribosomal entry site (EMCV)2IRES2internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP |
Ordering Information | |
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Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), human diphtheria toxin receptor (DTR) corresponds to the heparin binding EGF-like growth factor, Aequorea Victoria EGFP cDNA, Phage P1 loxP site, mouse Ly75 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System Immunology and Inflammation Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A, 109, 11288-11293 (2012).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes" specified by the DEPOSITOR and Inserm. |
Depositor | Katsuaki Sato (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |