Strain Information | |
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Image | |
BRC No. | RBRC05821 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Camk2a<tm1.1Oyam> |
Former Common name | B6-CaMKII alpha (K42R), B6-Camk2a KI/K42R |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Yoko Yamagata and Yuchio Yanagawa, National Institute for Physiological Sciences in 2003. Derived from TT2 ES cell line (B6CBAF1 derived). The PGK-neo cassette was removed by transient expression of cre recombinase in ES cells. C57BL/6 background (RBRC05821), ICR mixed background (RBRC05636). |
Strain description | Camk2a/K42R knock-in mice. Ca<2+>/calmodulin-dependent protein kinase IIalpha (CaMKII alpha/ Camk2a) is an enzyme that adds phosphates to a variety of protein substrates to modify their functions. CaMKIIalpha is especially enriched in the hippocampus, and is believed to be an essential mediator of activity-dependent synaptic plasticity and memory functions. We generated a novel kinase-dead knock-in mouse of the CaMKII alpha gene that completely and exclusively lacks its kinase activity by introducing a point mutation (K42R) that prevents ATP binding necessary for phosphorylation reaction. Both heterozygous and homozygous mice are hyperactive, but otherwise indistinguishable from wild-type mice. They are fertile and without any obvious neurological symptoms, except for sporadic observation of mild limbic seizure. Homozygous mice are severely impaired with hippocampal synaptic plasticity and hippocampus-dependent memory formation. Suitable for studies of the mechanisms of LTP and learning and memory, and as a memory-deficit animal model. |
Colony maintenance | Back crossed to C57BL/6NCrlCrlj for more than 20 generations. |
References | J. Neurosci., 29, 7607-7618 (2009). 19515929 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Camk2acalcium/calmodulin-dependent protein kinase II alpha18Camk2a<tm1.1Oyam>targeted mutation 1.1, Yoko Yamagata Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP |
Ordering Information | |
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Donor DNA | P1 Phage loxP site, mouse Camk2a genomic DNA |
Research application | Cre/loxP system Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 29, 7607-7618 (2009). |
Depositor | Yoko Yamagata (National Institute for Physiological Sciences) |
Strain Status | Frozen sperm |
Strain Availability | Live mouse (more than 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |