Strain Data Sheet

RBRC06211

Strain Information

Image
BRC No.RBRC06211
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Piga<tm1>
Former Common namePiga floxed, B6-Piga floxed
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Junji Takeda and Taroh Kinoshita, Research Institute for Microbial Diseases, Osaka University in 1997. R1 ES cells derived were used for the generation of floxed mice. The mutant mice were backcrossed to C57BL/6J over 10 times.
Strain descriptionPiga gene floxed mice. A loxP site was inserted between exons 5 and 6, and a loxP flanked Tk-neo cassette was inserted after the exon 5.
Colony maintenanceHeterozygote, Hemizygote x Wild-type [C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 94, 7400-7405 (1997). 9207103

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pigaphosphatidylinositol glycan anchor biosynthesis, class AXPiga<tm1Tak>targeted mutation 1, Junji Takeda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xherpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAPhage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Piga genomic DNA
Research applicationCre/loxP system
Hematological Research
Mouse Models for Human Disease
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorTaroh Kinoshita (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data