Strain Information | |
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Image | |
BRC No. | RBRC06436 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Pemt<tm1> |
Former Common name | B6-Pemt-/-, B6-Pemt KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Jun Wada, Okayama University Graduate School of Medicine, Eijiro Watanabe, Dainippon Sumitomo Phama Co. Ltd. C57BL/6 derived ES cells were used to generate the mutant mice. |
Strain description | Pemt gene knockout mice. Exon 2 of the Pemt gene was replaced with a neo cassette. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Biochimica et Biophysica Acta. 1831:626-632 (2013). 22877991 PLoS One 9(3):e92647, (2014). 24667182 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11 |
Ordering Information | |
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Donor DNA | Mouse PGK promoter (マウス由来ゲノムDNA), E. coli ネオマイシン リン酸塩基転移酵素(大腸菌由来cDNA), Mouse PGK poly A (マウス由来ゲノムDNA), Simian virus SV40 poly A (Simian virus 40由来ゲノムDNA),バクテリオファージ loxP配列(バクテイオファージP1由来ゲノムDNA) |
Research application | Cre/loxP system Diabetes and Obesity Research Metabolism Research Mouse Models for Human Disease covid19_progression |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Jun Wada (Okayama University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |