Strain Data Sheet

RBRC06589

Strain Information

Image
BRC No.RBRC06589
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Gt(ROSA)26Sor-C1V1/EYFP)1.1Imayo
Former Common nameR26 BAC::pCAG-LoxP-C1V1-EYFP, B6-ROSA26/C1V1-YFP/loxP Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi, Institute for Virus Research, Kyoto University in 2012. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionC1V1-EYFP(E122T/E162T) is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26 BAC. This modified BAC was used for Tg mouse production. To achieve high-level transgene expression, C1V1-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region. R26 BAC::pCAG-LF-C1V1-EYFP line (RBRC06588), R26 BAC::pCAG-LoxP-C1V1-EYFP line (RBRC06589) (FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain), R26 BAC::pCAG-FRT-C1V1-EYFP line (RBRC06590) (LoxP-Stop-LoxP cassette was removed by crossing TNAP-Cre strain).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe SorianoUNGt(ROSA)26Sor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
C1V1 (Chlamydomonas reinhardtii, Volvox carteri)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
beta-globin polyA signal (rabbit), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signalUN

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), Bacteriophage P1 loxP sites, mouse phosphoglucokinase (PGK) promoter, E. coli Neo, Bovine Growth hormone polyA signal, Rabbit beta-globin poly A signal, SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Yeast FRT (flippase recombination target) site, C1V1 (Chlamydomonas reinhardtii, Volvox carteri), Jellyfish EYFP cDNA, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), BAC containing mouse Rosa26 locus (RP23-401D9)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the C1V1・ChR2(SSFO) (Dr. Karl Deisseroth, https://web.stanford.edu/group/dlab/optogenetics/request_dna.html).
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Dr. Karl Deisseroth (English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data