Strain Information | |
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Image | |
BRC No. | RBRC09254 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Gt(ROSA)26Sor<tm1(CAG-kikGR)Kgwa> |
Former Common name | B6-ROSA/kikGR(floxed), B6-ROSA/kikGRfloxed KI |
H-2 Haplotype | |
ES Cell line | M1 [(C57BL/6 x 129)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Michio Tomura, Kyoto University Graduate School of Medicine. M1 ES cells were used to generate the mutant mice. |
Strain description | CAG-lox-STOP-lox-kikGR ROSA26 knock-in mice. C57BL/6 background (RBRC09254), BALB/c background (RBRC09255). |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Sci. Rep., e6030 (2014). 25112380 |
Health Report | |
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Examination Date / Room / Rack | 2022/05/09Room:2-検Rack:C |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-kikGR)Kgwa>targeted mutation 1, Osami Kanagawa Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter STOP6STOP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter kikGRGreen-Red Fluorescent Protein (Favia favus)6kikGRCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, Stop, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Favia favus Kikume-Green Red cDNA, chicken poly(A) sequence, mouse Gt(ROSA)26Sor genomic region |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., e6030 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the kikGR using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a MTA regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki. Osaka University (FAX:+81-6-6879-3829, e-mail: jimiyaza@nutri.med.osaka-u.ac.jp). The RECIPIENT must send the DEPOSITOR a reprint of the RECIPIENT's publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Haruhiko Koseki (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Feb 2017 |
BRC mice in Publications |
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No Data |