Strain Data Sheet

RBRC09701

Strain Information

Image
BRC No.RBRC09701
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Zkscan6<em2Ibbr>
Former Common nameB6. Zkscan6 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Fugaku Aoki and Helena Fulkova, The University of Tokyo in 2016. Fertilized eggs derived from C57BL/6N were used. C57BL/6N background.
Strain descriptionZkscan6 (zinc finger with KRAB and SCAN domains 6) mutant mice generated by the Genome editing technique (CRISPR/Cas9). This mutant contains 7 bp deletion at exon 3 of the Zkscan6 gene causing a frameshift. Homozygous mutants are viable and fertile. 2 bp deletion mutant of Zkscan6 is also available in RBRC09700.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zkscan6zinc finger with KRAB and SCAN domains 611Zkscan6<em2Ibbr>endonuclease-mediated mutation 2, Fugaku Aoki

Ordering Information

Donor DNA
Research application
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorFugaku Aoki (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data