Strain Data Sheet
RBRC10693
Strain Information | |
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| Image | |
| BRC No. | RBRC10693 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Nr3c1<tm1.1Himo> |
| Former Common name | GR loxP |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Hisashi Mori, University of Toyama in 2019. C57BL/6-derived ES cell line RENKA was used. A pgk-Neo cassette was removed by CAG-FLPe (RIKEN BRC, RBRC01834 ). C57BL/6 genetic background. |
| Strain description | Floxed Nr3c1 mouse.A pair of loxP flanks exon 3 of the Glucocorticoid receptor gene. |
| Colony maintenance | |
| References | eLife 7: e34135 (2018). 29941090 |
Health Report | |
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| Examination Date / Room / Rack | 2022/04/25Room:3-CRack:J |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Nr3c1nuclear receptor subfamily 3, group C, member 118Nr3c1<tm1.1Himo>targeted mutation 1.1, Hisashi Mori Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site18Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP |
Ordering Information | |
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| Donor DNA | Bacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT site, Mouse Nr3c1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. eLife 7: e34135, 2018.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application forany patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Hisashi Mori (University of Toyama) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |