Strain Information | |
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Image | |
BRC No. | RBRC10698 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Spp1<tm1.1(GFP)Yham> |
Former Common name | Sppl-GFP knock-in mouse |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Nagahiro Minato and Yoko Hamazaki, Kyoto University in 2015. Bruce 4 ES cells were used to generate this strain. B6 congenic line. |
Strain description | EGFP is knocked in at the translation initiation codon of Exon 2 of the Spp1 gene. |
Colony maintenance | |
References | J. Immunol., 194: 5725-5735 (2015). 25972477 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)5EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal5 |
Ordering Information | |
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Donor DNA | Aequorea victoria EGFP cDNA, Simian virus SV40 polyA, P1 Bacteriophage LoxP site, mouse Spp1 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 194: 5725-5735 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. (Contact : Medical Applications Promoting Office, Center for iPS Cell Research and Application, Kyoto University (CiRA) ) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Yoko Hamazaki (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |