Strain Information | |
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Image | |
BRC No. | RBRC10756 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Mab21l1<tm1Nao> |
Former Common name | C57BL/6(Mab21l1tm1Nao), Mab21l1tm1Nao |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | R1 ES cells were established in August 1991 from a male blastocyst hybrid of two 129 substrains (129X1/SvJ and 129S1/SV-+p+Tyr-c Kitl Sl-J/+).abnormal male preputial gland morphology abnormal eye morphology abnormal skull development |
Strain description | This gene is similar to the MAB-21 cell fate-determining gene found in C. elegans. It may be involved in eye and cerebellum development.Mutation details: The entire gene coding region was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutants was confirmed by whole mount in situ hybridization analysis of E10.5 homozygous mutant embryos. |
Colony maintenance | |
References | Development, 130(9), 1759-1770 (2003). 12642482 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)3herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA3 |
Ordering Information | |
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Donor DNA | 大腸菌(クラス1) Neomycin resistant gene, 単純ヘルペスウイルス1型(クラス2) Thymidine Kinase promoter, シミアンウイルス40(SV40)(クラス2) PolyA signal |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Naoki Takahashi (The University of Tokyo) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |