Strain Information | |
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Image | |
BRC No. | RBRC10852 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-Cst7<tm1Ike> |
Former Common name | CysF STOPtetO knockin |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Developed by Kazuhiro Ikenaka at National Institute for Physiological Sciences in collaboration with Kenji Sakimura at Niigata University. RENKA ES cells derived from C57BL/6N were used to generate this strain. C57BL/6N background. Homozygous mice are viable and fertile. |
Strain description | Knockin mice in which a STOP-tetO cassette was placed upstream of the translation initiation site of the Cst7 (Cystatin F) gene.Inserted DNA cassette contains loxP-FRT-Neo-poly A-FRT-tetO-loxP. |
Colony maintenance | |
References | Glia. 2017 Jun;65(6):917-930. doi: 10.1002/glia.23134. 28251676 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal2 |
Ordering Information | |
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Donor DNA | マウス PGK プロモーター, synthetic bacterial EM7 promoter (T7 phage), トランスポゾン Tn5由来 Neo 耐性遺伝子, tetracycline operator site 酵母 His3 5’ UTR, FRT SV40 polyA シグナル CMV 最小プロモーター バクテリオファージP1 loxP |
Research application | Cre/loxP system FLP/frt system Tet system |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Nobuhiko Ohno (National Institute for Physiological Sciences) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |