Strain Information | |
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Image | |
BRC No. | RBRC10897 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | C57BL/6J-Otx2<em1(tdTomato)Utr> |
Former Common name | Otx2-2A-tdTomato |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Fumihiro Sugiyama, University of Tsukuba in 2017. This strain was generated with the CRISPR/Cas9 using fertilized eggs derived from C57BL/6J. Homozygous mice are grossly normal and fertile. |
Strain description | Knockin mice harboring tdTomato at the end of translation initiation codon of the Otx2 gene with the P2A peptide. |
Colony maintenance | |
References | Exp. Anim., 2019 Jun 13. doi: 10.1538/expanim.19-0031. 31189761 |
Health Report | |
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Examination Date / Room / Rack | 2023/03/13Room:3-6Rack:B 2022/12/12Room:3-6Rack:B 2022/09/12Room:3-6Rack:B 2022/06/13Room:3-6Rack:B |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Otx2orthodenticle homolog 2 (Drosophila)14Otx2<em1(tdTomato)Utr>endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA14HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter P2Aporcine teschovirus-1 2A peptide14P2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tdTomatotandem dimeric Red Fluorescent Protein (Discosoma sp.)14tdTomato |
Ordering Information | |
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Donor DNA | P2A (porcine teschovirus-1 2A peptide), Discosoma sp. tdTomato cDNA, Rabbit Beta globin polyA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 2019 Jun 13. doi: 10.1538/expanim.19-0031. |
Depositor | Fumihiro Sugiyama (University of Tsukuba) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |