Strain Information | |
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Image | |
BRC No. | RBRC10920 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Gt(ROSA)26Sor<tm1(mVenus/Cdkn1b<*>)Toki> |
Former Common name | G0 marker mice |
H-2 Haplotype | |
ES Cell line | KY1.1[(C57BL/6J x 129S6/SvEvTac)F1] |
Background strain | |
Appearance | |
Strain development | Developed and deposited by Toshio Kitamura in 2019. ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Mice were further backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic line. |
Strain description | Knockin mouse strain at the ROSA26 locus in which mVenus-fused p27K can be overexpressed in a Cre-dependent manner. |
Colony maintenance | |
References | Cell Rep., 29(12):4144-4158 (2019). 31851939 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(mVenus/Cdkn1b<*>)Toki>targeted mutation 1, Toshio Kitamura Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SAadenovirus Splicing acceptor6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mVenusYellow Fluorescent Protein (Aequorea victoria)6mVenus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal6 |
Ordering Information | |
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Donor DNA | adenovirus Splicing acceptor, P1 phage LoxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistant gene, HSV tk polyA, Aequorea victoria mVenus cDNA, mouse p27K-(mutant p27) gene cDNA, SV40 virus PolyA signal, mouse Gt(ROSA)26Sor genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Toshio Kitamura (The University of Tokyo) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |