Strain Information | |
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Image | |
BRC No. | RBRC10928 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | C57BL/6J-Ucp1<em1(iRFP720)Utgr> |
Former Common name | Ucp1-iRFP720 KI mice |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Aya Fukuda, University of Tsukuba in 2017. This strain was generated with the CRISPR/Cas9 using fertilized eggs derived from C57BL/6J. Homozygous mice are fertile. |
Strain description | A reporter strain in which iRFP720 is placed around the translation initiation codon of the Ucp1 gene. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ucp1uncoupling protein 1 (mitochondrial, proton carrier)8Ucp1<em1(iRFP720)Utgr>endonuclease-mediated mutation 1, Koji Hisataka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)8GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBBhuman beta-globin intron8HBB Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter iRFP720Deinococcus radiodurans8iRFP720 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter human IgH intron8 |
Ordering Information | |
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Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mus musculus Ucp1 genomic DNA, Homo sapiens IgH intron, Homo sapiens beta-globin intron, Mus musculus Kozak sequence, Deinococcus radiodurans iRFP720, Bos taurus bGH polyA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) |
Depositor | Aya Fukuda (University of Tsukuba) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Feb 2021 |
BRC mice in Publications |
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No Data |