Strain Data Sheet

RBRC10949

Strain Information

Image
BRC No.RBRC10949
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-S1pr2<tm2(lacZ)Ytak>
Former Common nameS1PR2-LacZ knockin
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yo Takuwa, Kanazawa University in 2003. ES cells derived from 129 were used. C57BL/6 and 129 mixed genetic background.
Strain descriptionLacZ reporter strain for the S1pr2 gene. The coding exon was replaced with a lacZ and neo cassette.
Colony maintenance
ReferencesJ. Allergy Clin. Immunol., 132, 1205-1214 (2013). 24021572
PLoS One, 13, e0197604 (2018). 29782549
PLoS Biol, 15 (2), e2000949 (2017). 28248965
Cancer Res. 70(2):772-81 (2010). 20068174

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
S1pr2sphingosine-1-phosphate receptor 29S1pr2<tm2(lacZ)Ytkw>targeted mutation 2, Yoh Takuwa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal9

Ordering Information

Donor DNAE.coli Neomycin resistant gene, SV40 PolyA signal, E.coli LacZ
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Res. 2010 Jan 15;70(2):772-81. doi: 10.1158/0008-5472.CAN-09-2722
DepositorYoh Takuwa (Kanazawa University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data