Strain Information | |
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Image | |
BRC No. | RBRC10980 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6NJcl.Cg-Mdga2<tm1Tymo> |
Former Common name | Mdga2 KO |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Tohru Yamamoto, Faculty of Pharmaceutical Science, Hokkaido University. TT2 (B6CBF1 derived) ES cells were used to generate Mdga2 knockout mice. The mutant mice were backcrossed to C57BL/6N. C57BL/N congenic strain. |
Strain description | Mdga2 gene knockout mice. Exon 1 of the Mdga2 gene was replaced with lacZ and neo cassette. |
Colony maintenance | |
References | PLoS Genet. 2009 Jun;5(6):e1000536. 19557195 Neuron. 2016 Sep 7;91(5):1052-1068 27608760 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mdga2MAM domain containing glycosylphosphatidylinositol anchor 212Mdga2<tm1Tymo>targeted mutation 1, Toru Yamamoto Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site12Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site12Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)12 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox71mutant lox sequence12lox71 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)12mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | P1 phage lox71 site, E.coli LacZ, rabbit beta-globin 3’end, Yeast frt sites, mouse PGK promoter, E.coli neo, P1 phage loxP site, mouse PGK 3’end, mouse Mdga2 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0704K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Neuron. 91(5):1052-68.) Please inform theDEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | Tohru Yamamoto (Kagawa University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |