Strain Information | |
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Image | |
BRC No. | RBRC10983 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Hcn4<tm1(tTA)Nnka> Hcn4<tm1.1(Luc/EGFP)Nna> |
Former Common name | HCN4 tet/luc mice |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Noriyuki Nakashima and Makoto Takano, Kurume University in 2017. |
Strain description | This strain has two different alleles at the Hcn4 gene locus. One is a reporter allele in which luciferase/EGFP fusion gene was inserted in frame of translation initiation codon of the Hcn4 gene. Hcn4 gene is disrupted. The other is an allele in which tTA/Tre cassette was inserted at the translation initiation site of the Hcn4 gene. |
Colony maintenance | |
References | J. Physiol., 596(5):809-825 (2018). 29315578 |
Health Report | |
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Examination Date / Room / Rack | 2023/03/13Room:3-3Rack:D 2022/12/19Room:3-3Rack:D 2022/12/12Room:3-3Rack:D 2022/09/12Room:3-3Rack:D 2022/06/13Room:3-3Rack:D |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hcn4hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1.1(Luc/EGFP)Nna>targeted mutation 1.1, Noriyuki Nakashima Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hcn4hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1(tTA)Nnka>targeted mutation 1, Noriyuki Nakashima Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)9EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHhuman growth hormone gene9GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter URA3Orotidine-5'-phosphate (OMP) decarboxylase (yeast)9URA3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lucluciferase9luc Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)9tTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal9 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal9 |
Ordering Information | |
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Donor DNA | firefly Luciferase cDNA, jellyfish EGFP cDNA, Simian virus SV40, P1 phage loxP, Human human growth hormone gene, cytomegalovirus cytomegalovirus promoter, herpes simplex virus HSV thymidine kinase polyadenylation signal、HSV thymidine kinase promoter, E coli tetracycline transactivator、tet operator、neomycin resistant gene、yeast URA3 gene |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 596(5):809-825 (2018).For use of the BIOLOGICAL RESOURCE by a for-profit institution the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Makoto Takano (Kurume University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |