Strain Information | |
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Image | |
BRC No. | RBRC11137 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6J.B6N-Qrfp<tm2.1(icre)Stak> |
Former Common name | Qrfp-iCre |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Takeshi Sakurai, University of Tsukuba, 2020. Mice were generated using RENKA ES cells derived from C57BL/6NCrlCrlj. Mice were further backcrossed to C57BL/6J for 10 generations. Pgk-neo fragment was deleted from this strain. |
Strain description | iCre knock in mice at the translation initiation codon of the Qrfp gene. |
Colony maintenance | |
References | Nature. 2020 Jul;583(7814):109-114. doi: 10.1038/s41586-020-2163-6. 32528181 |
Health Report | |
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Examination Date / Room / Rack | 2023/01/23Room:3-CRack:G 2022/10/25Room:3-CRack:G 2022/07/25Room:3-CRack:G 2022/04/25Room:3-CRack:G |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Qrfppyroglutamylated RFamide peptide2Qrfp<tm2.1(icre)Stak>targeted mutation 2.1, Takeshi SakuraiP518, QRFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter icrephage P1 codon improved Cre (iCre)2icre |
Ordering Information | |
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Donor DNA | Bactriophage P1 Cre recombinase, SV40 poly A, Saccharomyces cerevisiae FRT site, mouse Qrfp genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature. 2020 Jul;583(7814):109-114. doi: 10.1038/s41586-020-2163-6. |
Depositor | Takeshi Sakurai (University of Tsukuba) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |