Strain Information | |
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Image | |
BRC No. | RBRC11193 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Gt(ROSA)26Sor<tm2(Asxl1<*>)Toki> |
Former Common name | Asxl1-MT KI |
H-2 Haplotype | |
ES Cell line | KY1.1[(C57BL/6J x 129S6/SvEvTac)F1] |
Background strain | |
Appearance | |
Strain development | Deposited by Toshio Kitamura in 2020. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Mice were further backcrossed to C57BL/6J for 6 generations. C57BL/6J congenic line. |
Strain description | Knockin mouse strain at the ROSA26 locus in which Asxl1-MT IRES EGFP can be overexpressed in a Cre-dependent manner. |
Colony maintenance | |
References | J. Exp. Med., 215(6):1729-1747 (2018). 29643185 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2(Asxl1<*>)Toki>targeted mutation 2, Toshio Kitamura Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)6FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 virus Stop6 |
Ordering Information | |
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Donor DNA | SA (Adenovirus Splicing acceptor), P1 phage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, SV40 virus Stop, FLAG tag (synthetic), Mouse Asxl1 (1-1,890) cDNA, yeast FRT (flippase recombination target) sites, IRES (internal ribosomal entry site (EMCV)), jellyfish EGFP cDNA, bovine growth hormone polyA, mouse Gt(ROSA)26Sor genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Exp Med. Jun 4;215(6):1729-1747 (2018)For use of the BIOLOGICAL RESOURCE by a company and a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Toshio Kitamura (The University of Tokyo) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |