Strain Information | |
---|---|
Image | |
BRC No. | RBRC11202 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | STOCK Slc12a2<tm1.1(tetO)Kftnk> |
Former Common name | NKCC1 tetO knockin |
H-2 Haplotype | |
ES Cell line | G0G1 |
Background strain | |
Appearance | |
Strain development | Deposited by Kenji Takana, Keio University in 2021. Mixed genetic background. |
Strain description | TetO cassette knock in at the Slc12a2 genomic area between its promoter and coding sequence. |
Colony maintenance | |
References | Nat. Commun., 12(1) 5146 (2021). 34446732 Sci. Rep., 7(1):13605. (2017). 29051615 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Slc12a2solute carrier family 12, member 218Slc12a2<tm1.1(tetO)Kftnk>targeted mutation 1.1, Kenji Tanaka Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site18Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tetOtetracycline operator (E. coli)18tetO |
Ordering Information | |
---|---|
Donor DNA | phage P1 loxP sites, yeast FRT (flippase recombination target) site, tetracycline operator (E. coli), Cytomegalovirus CMV minimum promoter, mouse Slc12a2 genomic DNA |
Research application | Cre/loxP system FLP/frt system Tet system |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Kenji Tanaka (Keio University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |