Strain Data Sheet

RBRC11204

Strain Information

Image
BRC No.RBRC11204
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Adipoq<em1(tdTomato)Gifim>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Hiroyuki Morita and Kazuo Kajita, Gifu University in 2020. Mice were generated using C57BL/6J with the CRISPR/Cas9.
Strain descriptionKnock in mice expressing tdTomato from the Adipoq gene locus.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Adipoqadiponectin, C1Q and collagen domain containing16Adipoq<em1(tdTomato)Gifim>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide16P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tdTomatotandem dimeric Red Fluorescent Protein (Discosoma sp.)16tdTomato

Ordering Information

Donor DNAP2A (porcine teschovirus-1 2A peptide), Discosoma sp. tdTomato cDNA, mouse gRNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 1. The availability of the BIOLOGICAL RESOURCE is restricted to a RECIPIENT of a not-for profit organization for a not-for-profit research. 2. For use of the BIOLOGICAL RESOURCE by a for-profit organization and by a not-for profit organization for a for-profit research, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 3. RECIPIENT must contact the DEPOSITOR In the case of application for any patents or for-profit use based on the results from the use of the BIOLOGICAL RESOURCE. 4. Prior to requesting the BIOLOGICAL RESOURCE the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroyuki Morita (Gifu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (TBUSAMTA_Nonprofit_BROAD (non-US)) / (TBUSA_Broad_lisence (US))
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data