Strain Information | |
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Image | |
BRC No. | RBRC11223 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | STOCK Del(2Cstl1-Cstdc2)1Osb |
Former Common name | (Cstl1-Cstdc2)<del/wt> |
H-2 Haplotype | |
ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background. |
Strain description | Deletion mutant of 114 kb on chromosome 2 covering Cstl1, Cst11, Cstdc1, Cst12, Cst8, Cst13, Cst9 and Cstdc2. |
Colony maintenance | |
References | Proc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). doi: 10.1073/pnas.1908736116. Epub 2019 Aug 27 31455729 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter |
Ordering Information | |
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Donor DNA | human hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Cstl1 gene,Mouse a part of Cstdc2 gene |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506. doi: 10.1073/pnas.1908736116. Epub 2019 Aug 27RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |