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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Research tools to investigate the role of PD-1 in the mouse


Congratulations to Distinguished Professor Tasuku Honjo for the 2018 Nobel Prize.


The 2018 Nobel Prize in Physiology or Medicine was awarded jointly to Drs. Tasuku Honjo and James P. Allison “for their discovery of cancer therapy by inhibition of negative immune regulation” [1]. Dr. Honjo and his colleagues at Kyoto University discovered PD-1, a protein expressed on the surface of T-cells, which functions as a T-cell brake [2-5]. They also revealed antibodies against PD-1 inhibit function of the brake, leading to activation of T-cells and efficient attack to cancer cells in animal experiments including those using mutant mice which they kindly deposited to RIKEN BRC [6]. Their mouse strains useful for understandng immune system as well as the role of PD-1 have been distributed to over 200 institutions worldwide and have contributed to establish immune checkpoint therapy, which has dramatically changed the outcome of certain portion of patients with cancer.

Popular strains

RBRC No.Strain name
RBRC00903C.129S2-Pdcd1<tm1Hon>/HonRbrc (N10)
RBRC00904C.129S2-Pdcd1<tm1Hon>/HonRbrc (N12)
RBRC02142B6.129S2-Pdcd1<tm1Hon>/HonRbrc
RBRC02361NOD.129S2(B6)-Pdcd1<tm1Hon>
RBRC02363C.129S2-Pdcd1<tm1Hon>(N20)
RBRC02364MRL.129S2(B6)-Pdcd1<tm1Hon>
RBRC02365C57BL/6-Tg(Actb-Pdcd1)BHon



References
[1] The 2018 Nobel Prize in Physiology or Medicine
https://www.nobelprize.org/prizes/medicine/2018/
[2] Ishida Y, Agata Y, Shibahara K, Honjo T. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 11(11):3887-95.
[3] Nishimura H, Nose M, Hiai H, Minato N, Honjo T. (1999) Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 11(2):141-51.
[4] Freeman GJ, Long AJ, Iwai Y, Bourque K, Chernova T, Nishimura H, Fitz LJ, Malenkovich N, Okazaki T, Byrne MC, Horton HF, Fouser L, Carter L, Ling V, Bowman MR, Carreno BM, Collins M, Wood CR, Honjo T. (2000) Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 192(7):1027-34.
[5] Nishimura H, Okazaki T, Tanaka Y, Nakatani K, Hara M, Matsumori A, Sasayama S, Mizoguchi A, Hiai H, Minato N, Honjo T. (2001) Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice. Science 291(5502):319-22.
[6] Iwai Y, Terawaki S, Honjo T. (2005) PD-1 blockade inhibits hematogenous spread of poorly immunogenic tumor cells by enhanced recruitment of effector T cells. Int Immunol. 17(2):133-44.



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Other strains

RBRC No.Strain name
RBRC00889MINT-KO (ICR background)
RBRC00891MINT-KO (C57BL/6 background)
RBRC00892AID-conditional Transgenic mouse line 20
RBRC00893AID-conditional Transgenic mouse line 4
RBRC00894AID-Tg line #B1
RBRC00895AID-Tg line #B2
RBRC00896AID-Tg line #C1
RBRC00897B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc
RBRC00898B6.Cg-Aicda<tm1Hon>/HonRbrc
RBRC00901C.Cg-Aicda<tm1Hon>/HonRbrc (N10)
RBRC01071Floxed RBP-J
RBRC01101B6.129S2-Rbpj<tm1Kyo>/HonRbrc
RBRC01234C57BL/6-Tg(H2K-PDL1)1Hon/HonRbrc
RBRC02103B6.Cg-Spen<tm2>/HonRbrc
RBRC02104B6.Cg-Spen<tm2.1Hon> Rbpj<tm1Hon>/HonRbrc
RBRC02105B6.Cg-Spen<tm2.1>/HonRbrc
RBRC02393C.Cg-Aicda<tm1Hon>(N12)
RBRC04892B6(Cg)-Aicda<tm2.1Hon>
RBRC05550B6.Cg-Ighm<tm1Cgn> Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Sho>
RBRC05551B6.Cg-Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Hjf>
RBRC05938C57BL/6-Tg(Aicda*dME-cre,-CD2)1Hon
RBRC06296CAJcl.Cg-Aicda<tm2.1Hon>
RBRC06297CAJcl.Cg-Aicda<tm1Hon>
RBRC06298CAnPt.Cg-Aicda<tm2.1Hon>
RBRC06299CAnPt.Cg-Aicda<tm1Hon>
RBRC06300CAnPt.Cg-Aicda<tm1Hon>/+