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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

JoVE 3155 11/18/2011

RIKEN BioResource Center Mouse E-mail Message No. 33

RIKEN BioResource Center provides you the most up-to-date information
such as newly deposited mouse strains.

=======  content  ======================================================
- Special topic: Mouse of the Month
- Newly deposited mouse strains
- Newly available mouse strains
- Mouse strains for freeze preservation
- Visit from Lanzhou Institute of Biological Products
- BRC mice in publication

|  Special topic: Mouse of the Month    Feb 2007

  FLPe transgenic mouse: A tool for conditional genetic manipulation

         Depositor: Dr. Shigeyoshi Itohara (RIKEN BSI)
            BRC No. 01834_B6-Tg(CAG-FLPe)36
            BRC No. 01835_B6-Tg(CAG-FLPe)37

Mouse of the Month archive

|  Newly deposited mouse strains

  These mice have been newly deposited to RIKEN BRC. They will be
available soon after rederivation. We appreciate depositors for their
contributions to the scientific community.

Reg#        Strain Name                            Depositor
01940       C3H/HeN-11<NSY>                        Dr. Hiroshi Ikegami
02077       Slp1 KO mouse                          Dr. Mitsunori Fukuda
02078       Slp2-a KO mouse                        Dr. Mitsunori Fukuda
02095       B6.Cg-Tg(TH-GFP)21-31                  Dr. Kazuto Kobayashi
02096       B6.Cg-Tg(CAG-floxed Neo-EGFP)REP08Osb  Dr. Masaru Okabe
02097       B6;129Sv-Izumo(tm1Osb)                 Dr. Masaru Okabe
02098       Plexin-A2 KO                           Dr. Hajime Fujisawa
02099       Plexin-A4 KO                           Dr. Hajime Fujisawa
02100       SYC-SB16                               Dr. Junji Takeda
02102       Arpc3 KO                               Dr. Junji Takeda
02106       B6.129-lefty1<tm1Hmd>                  Dr. Hiroshi Hamada
02107       B6.129-nodal<tm1Hmd>                   Dr. Hiroshi Hamada
02108       B6;C3;129-Tg(nodal ASE-lacZ)H8Hh       Dr. Hiroshi Hamada
02109       B6;129-Gmcl1<tm1Nak>                   Dr. Toru Nakano
02110       Plexin-A3 -flox mouse                  Dr. Hajime Fujisawa
02111       Plexin-A3 knockout mouse               Dr. Hajime Fujisawa

|  Newly available mouse strains

  These mice just have been rederived into our SPF barrier facility.
These mice are now available for distribution.

Reg#        Strain Name                            Depositor
01237       KAZ/TUA                                Dr. Kimiyuki Tsuchiya
01667       SM.A-T2dm3saR4                         Dr. Fumihiko Horio
01722       SM.A-T2dm3saR1A                        Dr. Fumihiko Horio
01816       D2R-IL2R                               Dr. Kazuto Kobayashi
01817       D4R-IL2R                               Dr. Kazuto Kobayashi
01828       B6.Cg-Tg(CAG-cre)CZ-MO2Osb             Dr. Masaru Okabe
01834       B6-Tg(CAG-FLPe)36                      Dr. Shigeyoshi Itohara
01835       B6-Tg(CAG-FLPe)37                      Dr. Shigeyoshi Itohara
01840       GluRε4 KO mouse                        Dr. Masayoshi Mishina
01854       B6.CS-Ngu1143                          Dr, Shizufumi Ebihara
01858       TUBG2 Knockout mouse                   Dr. Akiko Kubo
01859       FVB-Tg(BOS-inv/egfp)B27Hh              Dr. Hiroshi Hamada
01860       FVB-Tg(BOS-inv/egfp)N105Hh             Dr. Hiroshi Hamada
01861       C57BL/6-Tg(NodalNDE-Cre)16Hh           Dr. Hiroshi Hamada

|  Mouse strains for freeze preservation

  Since enough number of embryos has been preserved, live mice of these
strains will be closed soon. It will take more than 3 months to recover
live mice from frozen stock. Please let us know as soon as possible, if
you plan to use the mice and request them alive.

|  Visit from Lanzhou Institute of Biological Products

  An assistant manager for the animal facility of Lanzhou Institute of
Biological Products visited RIKEN BRC on Jan 15th, and stayed for a month
to learn advanced technology for management of SPF animal facility.

|  BRC mice in publication

  The research progress has been published on Nature Methods by using
the mice distributed from RIKEN BRC.

User  Dr. Takashi Tsuji (Tokyo University of Science)
BRC No. 00267_ C57BL/6-TgN(act-EGFP)OsbC14-Y01-FM131

  RIKEN BRC needs to demonstrate publicly our contribution to the
scientific community by disclosing our user's research publications.
We would like to request a reprint of any published research paper
involved the BIOLOGICAL RESOURCE supplied by RIKEN BRC. We really
appreciate your cooperation for sake of our sustainable activity and
the advancement of science.

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*These contents are subject to change without notice.

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RIKEN BioResource Center
Experimental Animal Division
3-1-1 Koyadai, Tsukuba-shi
Ibaraki, 305-0074 Japan