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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

JoVE 3155 11/18/2011

RIKEN BioResource Center Mouse E-mail Message No. 74

RIKEN BioResource Center (BRC) is pleased to update you with 
the latest information.

=======  contents  =====================================================
- Special topic: Mouse of the Month 
  "A Systemic Inflammation Model:Unc93B1 with a D34A mutation"
- Suspension of operation at RIKEN BRC for three days in this summer,
  from August 22 to August 24, 2011
- User publications
- Newly deposited mouse strains
- Newly available mouse strains
- Mouse strains for freeze preservation

|  Special topic: Mouse of the Month    July. 2011

  A Systemic Inflammation Model:Unc93B1 with a D34A mutation
    RBRC04929  B6;129P2(129)-Unc93b1<tm1.1(D34A)Kmiy>
    RBRC04930  B6.129P2(129)-Unc93b1<tm1.1(D34A)Kmiy>
    RBRC04931  C.129P2(Cg)-Unc93b1<tm1.1(D34A)Kmiy>
    Depositor:Dr. Kensuke Miyake, The Institute of Medical Science,
              The University of Tokyo

  Mouse of the Month archive


  The reproduction technology is required for stable and efficient
preservation of mouse strains, and the convenient transportation.
The overall data on survival and birth rates from frozen embryos in each
mouse strain become available at the web site of the Experimental Animal
Technical transfer to other laboratories is also our important mission.
We will hold "the 9th technical training course for cryopreservation of
mouse embryos and sperm" in coming October. Please contact us for any
questions by e-mail.

By Keiji Mochida, Bioresource Engineering Division

Cryopreservation Recovery Test Results

Lab HP (Bioresource Engineering Division)

|  Suspension of operation at RIKEN BRC for three days in this summer,
|  from August 22 to August 24, 2011

  To cooperate with the sever shortage of electricity supply expected in
this summer due to the Eastern Japan Disaster, RIKEN will close its all
campuses and facilities for three days, from Monday, August 22 to
Wednesday, August 24. During the period, RIKEN BRC will also suspend its
all operation, including shipments of bioresources and responses to the
orders and inquiries.
  All operation at RIKRN BRC will be conducted normally until Friday,
August 19 and from Thursday, August 25.
  This suspension may cause some inconvenience to our users. We
sincerely ask you for your understanding and cooperation.

|  User publications

  The following publications have been newly reported by users. We are
honored to continue serving the scientific community by introducing new
publications. Therefore, we look forward to receiving your publications

RBRC00805  Hox C cluster Knockout mice
Tschopp P, Fraudeau N, Béna F, Duboule D. Reshuffling genomic landscapes
to study the regulatory evolution of Hox gene clusters. Proc Natl Acad
Sci U S A.;108(26):10632-7. 2011

00984  ICR;129P2-Nfe2l2<tm1Mym>/MymRbrc
Clarke JD, Hsu A, Williams DE, Dashwood RH, Stevens JF, Yamamoto M, Ho E.
Metabolism and Tissue Distribution of Sulforaphane in Nrf2 Knockout and
Wild-Type Mice. Pharm Res. 2011 Jun 17.

00438  SEK1 Knockout mice
Sugiyama D, Kulkeaw K, Mizuochi C, Horio Y, Okayama S. Hepatoblasts
comprise a niche for fetal liver erythropoiesis through cytokine
production. Biochem Biophys Res Commun.;410(2):301-6.

02290  STOCK Tg(Nanog-GFP,Puro)1Yam
Araki R, Hoki Y, Uda M, Nakamura M, Jincho Y, Tamura C, Sunayama M, Ando
S, Sugiura M, Yoshida MA, Kasama Y, Abe M. Crucial Role of c-Myc in the
Generation of Induced Pluripotent Stem Cells. Stem Cells. 2011 Jul 5.
doi: 10.1002/stem.685.

|  Newly deposited mouse strains

  The following mice have been newly deposited into RIKEN BRC and 
will be available shortly after rederivation.
We appreciate the contributions of the depositors to the scientific

  Dr. Naoki Yamamoto, Fujita Health University
    RBRC02889  D2.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Ny

  Dr. Ichiro Sora, Tohoku university
    RBRC04750  C57BL/6J-Tg(DBH-hMOR)B
    RBRC04751  C57BL/6J-Tg(DBH-hMOR)C

  Dr. Tasuku Honjo, Graduate School of Medicine, Kyoto University
    RBRC04892  B6(Cg)-Aicda<tm2.1Hon>

  Dr. Teizo Fujita, Fukushima Medical University
    RBRC04883  B6.129-Fcnb<tm1Tefu>

  Dr. Shigekazu Nagata, Osaka University Graduate School of Medicine,
  Faculty Medicine
    RBRC04895  B6(Cg)-Timd4<tm1Osa>

  Dr. Haruhiko Koseki, RIKEN Research Center For Allergy and Immunology
    RBRC04848  B6.B6129-Gt(ROSA)26Sor<tm1(CAG-ECFP/Venus)Kgwa>

  Dr. Kuniya Abe, RIKEN BioResource Center
    RBRC02841  B6;B6D2-Tg(Oct4deltaPE-KikumeGR)2Rbrc
    RBRC04946  B6N.MSM-(D17Mit16-D17Mit31)/Rbrc
    RBRC02833  B6;B6D2-Tg(Oct4deltaPE-KikumeGR)1Rbrc

|  Newly available mouse strains

  The following mice have recently been rederived into our barrier
facility and are now available for distribution.

    RBRC04496  B6.129S1-Sept9<tm2Emfu>
    RBRC04707  STOCK Tg(Kcnc1-EYFP)27Bsi Gad1<tm1Tama>
    RBRC04706  STOCK Tg(aMHC-VSFP2.3)123Bsi
    RBRC04747  STOCK Tg(aMHC-GCaMP2)60Bsi
    RBRC04805  C57BL/6-Sept8<tm1.1>
    RBRC02605  TD1
    RBRC04359  C57BL/6J-Tg(hSMA-TRPC3DN)1
    RBRC04545  129-Rnf135<tm1>
    RBRC04739  B6.B6CB-Ltb4r1<tm1Tksh>
    RBRC04740  C.B6CB-Ltb4r1<tm1Tksh>
    RBRC04741  B6.B6CB-Ltb4r2<tm1Tksh>
    RBRC04719  B6;129B6-Qilin<tm1.1Hmd>
    RBRC03451  pOCT-GFP/BR Tg
    RBRC04778  B6.129S2-Cspg5<tm3Aon>/1Idr
    RBRC04779  B6.129S2-Cspg5<tm3Aon>/2Idr

|  Mouse strains for freeze preservation

  Since a sufficient number of embryos have been preserved,
live mice of the following strains will be discontinued soon.
It will take more than 3 months to recover live mice from frozen stock. 
Therefore, please inform us as soon as possible if you plan to use the 
mice and request them alive.

    RBRC02388  B6.129P2-Ly96<tm1Kmiy>
    RBRC01190  Evi9/BclIIa Knockout mice
    RBRC02889  D2.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Ny
    RBRC01662  mGluR1 KO
    RBRC01544  B6.129P2-Sirpa<tm1Nog>/Rbrc

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*If you have any questions or comments, please send an e-mail to

*Content is subject to change without notice.

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RIKEN BioResource Center
Experimental Animal Division
3-1-1 Koyadai, Tsukuba-shi
Ibaraki, 305-0074 Japan