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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

JoVE 3155 11/18/2011

RIKEN BioResource Center Mouse E-mail Message No. 79

RIKEN BioResource Center (BRC) is pleased to update you with the latest

=======  contents  =====================================================
- Special topic: Mouse of the Month
  "Neurodegeneration via endoplasmic reticulum stress"
- Video protocol, cryopreservation of mouse embryos appears on line
- Report: The 6th AMMRA and 7th SALAS Regional Conference, Singapore
- User publications
- Our recent publication
- Newly available mouse strains
- Mouse strains for freeze preservatio

|  Special topic: Mouse of the Month    Dec. 2011

Neurodegeneration via endoplasmic reticulum stress
    RBRC05350  B6;B6D2-Tg(Thy1-BSCL2*N88S)1
    Depositor:Dr. Daisuke Ito, Keio University

Mouse of the Month archive

|   Video protocol, cryopreservation of mouse embryos appears on line

Video protocol, "Cryopreservation of Mouse Embryos by Ethylene
Glycol-Based Vitrification" appears on line in the Journal of Visualized
Experiments, JoVE.

Watch the video

|  Report: The 6th AMMRA and 7th SALAS Regional Conference, Singapore

The 6th Asian Mouse Mutagenesis and Resource Association (AMMRA) council
meeting was held on November 29, 2011 at the Matrix Building, Biopolis,
Singapore. Subsequently, the 7th Singapore Association for Laboratory
Animal Science (SALAS) and 6th AMMRA Regional Conference 2011 were
jointly held on November 30-December 2 at the Concorde Hotel, Singapore.

|  User publications

  The following publications have been newly reported by users. We are
honored to continue serving the scientific community by introducing new
publications. Therefore, we look forward to receiving your publications

RBRC01351  C57BL/6-Grm2 tm
Fribourg M, Moreno JL, Holloway T, Provasi D, Baki L, Mahajan R, Park G,
Adney SK, Hatcher C, Eltit JM, Ruta JD, Albizu L, Li Z, Umali A, Shim J,
Fabiato A, Mackerell AD Jr, Brezina V, Sealfon SC, Filizola M,
Gonz lez-Maeso J, Logothetis DE.
Decoding the Signaling of a GPCR Heteromeric Complex Reveals a Unifying
Mechanism of Action of Antipsychotic Drugs. Cell; 147 (5), 1011-1023,

RBRC01828  B6.Cg-Tg(CAG-Cre)CZ-MO2Osb
Kobayashi M, Sabouri Z, Sabouri S, Kitawaki Y, Pommier Y, Abe T,
Kiyonari H, Honjo T.
Decrease in topoisomerase I is responsible for activation-induced
cytidine deaminase (AID)-dependent somatic hypermutation. Proc Natl Acad
Sci USA.;108(48):19305-19310. Epub 2011 Nov 11.

RBRC00806  GFP-LC3#53
T Yoshizaki, C Kusunoki, M Kondo, M Yasuda, S Kume, K Morino, O Sekine,
S Ugi, T Uzu, Y Nishio, A Kashiwagi, H Maegawa.
Autophagy regulates inflammation in adipocytes. Biochem. Biophys. Res.
Commun., 2011.

RBRC00806  GFP-LC3#53
Paul G. Thomes, Casey S. Trambly, Geoffrey M. Thiele, Michael J. Duryee,
Howard S. Fox, James Haorah, Terrence M.
Donohue Jr. Proteasome activity and autophagosome content in liver are
reciprocally regulated by ethanol treatment. Biochem. Biophys. Res.
Commun., 2011.

RBRC01733  Platelet-activating factor (PAF) receptor knockout mouse
Tanaka Y, Nagai Y, Kuroishi T, Endo Y, Sugawara S.
Stimulation of Ly-6G on neutrophils in LPS-primed mice induces
platelet-activating factor (PAF)-mediated anaphylaxis-like shock.
J Leukoc Biol. 2011 Nov 30. [Epub ahead of print]

RBRC01888  C57BL/6-Wsh/Wsh
Murata T, Aritake K, Matsumoto S, Kamauchi S, Nakagawa T, Hori M,
Momotani E, Urade Y, Ozaki H.
Prostagladin D2 is a mast cell-derived antiangiogenic factor in lung
carcinoma. Proc Natl Acad Sci USA. 2011 Nov 21. [Epub ahead of print]

|  Our recent publication

Mochida K, Hasegawa A, Taguma K, Yoshiki A, Ogura A. Cryopreservation of
mouse embryos by ethylene glycol-based vitrification. J Vis Exp.;(57),
2011. pii: 3155. doi: 10.3791/3155.

|  Newly available mouse strains

  The following mice have recently been rederived into our barrier
facility and are now available for distribution.

    RBRC04813  B6.B6D2-Tg(Eno2-IREB2)1Kiwa
    RBRC04864  B10.B6-Dct<slt>/Hir
    RBRC04887  B6.Cg-Cacna1a<tm1.1(CACNA1A)Ttan>
    RBRC04888  B6.Cg-Cacna1a<tm2.1(CACNA1A)Ttan>
    RBRC04938  B6.B6D2-Tg(A1AT-Spp1)25

|  Mouse strains for freeze preservation

  Since a sufficient number of embryos have been preserved, live mice of
the following strains will be discontinued soon. 
It will take more than 3 months to recover live mice from frozen stock.
Therefore, please inform us as soon as possible if you plan to use the
mice and request them alive.

    RBRC01190  Evi9/BclIIa Knockout mice
    RBRC01260  Xist1loxGFPmice
    RBRC01663  K14-AhR-CA TG mouse line 239
    RBRC01897  B6.129P2-Sox2<tm1Okud>
    RBRC02390  B6.129P2-Cd180<tm1Kmiy>
    RBRC02591  GIT2(-) (Acc. No. CDB0414K)
    RBRC02605  TD1
    RBRC02889  D2.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Ny
    RBRC03829  STOCK Tg(Lefty1 9.5-lacZ)F32Hmd
    RBRC03981  ICR;B6C3-Tg(3xFLAG-Mili)1Ysa
    RBRC04058  C57BL/6-Tg(Nes-d4YFP*)1HOkn
    RBRC04359  C57BL/6J-Tg(hSMA-TRPC3DN)1
    RBRC04360  C57BL/6J-Tg(hSMA-TRPC6)1
    RBRC04361  C57BL/6J-Tg(hSMA-TRPC6DN)1
    RBRC04417  B6;129-Sap35<tm1.1Hmd>/226
    RBRC04422  C57BL/6-Tg(CAG-Nup85)1-8Sush
    RBRC04609  B6;129P2-Wnt5a<tm1.1Homy>
    RBRC04885  B6;129S4-Cacna1e<tm1Ttan>

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RIKEN BioResource Center
Experimental Animal Division
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