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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Aug 2009 Visualizing the Wnt signal pathway


mouse_of_month_200908

Visualizing the Wnt signal pathway

TOP-GAL RBRC 02228
mn0908_0101

Visualization of the Wnt canonical signaling pathways in the TOP-GAL (RBRC02228) by X-gal staining at embryonic days 10.5.

 

  Wnt proteins belong to a family of secreted and lipid-modified glycoproteins that can activate intracellular signaling pathways, such as the Wnt/planar cell polarity, the Wnt/calcium and the Wnt β-catenin-or so-called Wnt canonical-signaling pathways. The Wnt canonical pathway during embryogenesis has been intensively studied. However, the role of this pathway in neonatal and adult tissues is unknown. TOP-GAL (RBRC02228) transgenic mouse is a reporter line that can visualize the Wnt canonical pathway by β-galactosidase activity in adult tissues. This transgenic mouse line was generated with ins-TOPGAL that accurately drives β-galactosidase expression under TCF/β-catenin and will provide you unique opportunities to investigate the function of Wnt canonical pathwayin vivo.

 

Depositor : Professor Yumiko Saga, National Institute of Genetics, Mishima, Japan
Reference : Genesis. 2007 Feb;45(2):90-100.