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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Feb 2007 FLPe transgenic mouse


mouse_of_month_200702

FLPe transgenic mouse
A tool for conditional genetic manipulation

BRC No. 01834_B6-Tg(CAG-FLPe)36
BRC No. 01835_B6-Tg(CAG-FLPe)37
mn0702_0101
BRC No. 01834_B6-Tg(CAG-FLPe)36

FLP-FRT system: Floxed mice can be generated after excision of neo (selective marker gene) by crossing with FLP Tg mice.

 

    The FLP-FRT system is similar to the cre-loxP system and is useful for more complex dual approaches that combine FLP with other recombinases, such as Cre and ΦC31.. FLPe is a variant of the FLP recombinase with a higher enzymatic activity at 37°C. This transgenic mouse of C57BL/6 strain expresses the FLPe recombinase under the control of the CAG promoter. This strain can be used as ‘FLP deleter’ mouse by crossing with the other genetically-engineered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest.

 

Depositor : Shigeyoshi Itohara (RIKEN BSI)
Reference : Kanki H, Suzuki H, Itohara S.
High-efficiency CAG-FLPe deleter mice in C57BL/6J background.
Exp. Anim., 55, 137-141 (2006).