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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Feb 2012 Green congenic mouse strains


mouse_of_month

Green congenic mouse strains

C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb  RBRC00267

C.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc  RBRC01624

C3.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc  RBRC01625

MSM.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc  RBRC01626

D2.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/NyRbrc  RBRC02889

mn1202_0101

Dorsal view of green C57BL/6 mice at 17 days old under (1A) white illumination and (1B) epi-fluorescence illumination. Green BALB/c mice at 17 days old under (1C) white illumination and (1D) epi-fluorescence illumination. Fluorescence in the skin is less visible in Black mice than in albino mice. Green fluorescence can be detected during different developmental stages, such as (2A) a 2-cell stage embryo, (2B) a 10-day embryo, and (2C) a newborn pup.

 

The green mouse strain was originally generated using the C57BL/6 background and expresses enhanced green fluorescent protein (EGFP) under the control of a chicken beta-actin promoter and cytomegalovirus enhancer (CAG promoter) [1]. The green mouse has been widely used in various developmental studies to visualize cell fate in vivo [1-3]. Recently, there has been an increasing demand from researchers for this in vivo fluorescent marker system to be extended to other useful inbred strains. To meet these requests, original C57BL/6-Tg(CAG-EGFP) mice were backcrossed with BALB/cAn, C3H/HeN, DBA/2Cr, and MSM/MsRbrc inbred strains to establish green mice in five different inbred strains. The genetic background of each strain was then examined using simple sequence length polymorphic markers to assess congenic status. A genotyping protocol was also established to distinguish homozygous and hemizygous transgenic mice. These green congenic mice will be useful for various cell transplantation studies to monitor the fate of donor cells in different genetic backgrounds.

Depositors:

Dr. Masaru Okabe, Osaka University

C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb RBRC00267

Dr. Masaru Okabe and RIKEN BioResource Center

C.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc RBRC01624
C3.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc RBRC01625
MSM.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc RBRC01626

Dr. Naoki Yamamoto, Fujita Health University

D2.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/NyRbrc RBRC02889

References:

  1. Ikawa, M., Yamada, S., Nakanishi, T., and Okabe, M. 1999. Green fluorescent protein (GFP) as a vital marker in mammals. Curr Top Dev Biol 44: 1-20.
  2. Kato, M., Yamanouchi, K., Ikawa, M., Okabe, M., Naito, K., and Tojo, H. 1999. Efficient selection of transgenic mouse embryos using EGFP as a marker gene. Mol Reprod Dev54: 43-48.
  3. Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T., and Nishimune, Y. 1997. ‘Green mice’ as a source of ubiquitous green cells. FEBS Lett 407: 313-319.