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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Sep 2005 Visualization of Primordial Germ Cells in Vivo


September 2005
Mouse of the Month

Visualization of Primordial Germ Cells in Vivo

Transgenic Model
Specific Expression of Oct4/GFP in Germ Cells
BRC No. 00821Strain name:TgN(deGFP)18Imeg
mn050927_0101 mn050927_0102
mn050927_0103 mn050927_0104

 

Strain description: Developed by Dr. Kuniya Abe (1999). The GOF-18/delta PE/EGFP plasmid was injected into the pronucleous of C57BL/6 fertilized eggs. GOF18ΔPE was prepared from Oct4 18-kb genomic fragment by deletion in the proximal enhancer (ΔPE). The Oct4 gene is known to be expressed in primordial germ cells and undifferenciated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.

 

DEPOSITOR: Dr. Kuniya Abe. Technology and Development Team for Mammalian Cellular Dynamics, RIKEN BRC.

 

Further information: abe@brc.riken.jp