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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Sep 2006 Thy1-SpH transgenic mouse


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Thy1-SpH transgenic mouse
Visualize vesicular exocytosis in brain

BRC No. 01482-01485, 01519 and 01520
Thy1-SpH transgenic mice series
Research Application : Neurobiology
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BRC No. 01519_Thy1-SpH transgenic mice (TV-42)
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Exocytotic SpH fluorescence at individual synapses

 

  Vesicular exocytosis at presynaptic terminals is fundamental function for neurotransmission and brain function. The lumen of a secretory vesicle is acidic (pH 5.6), whereas it becomes neutral (pH 7.4) instantaneously upon exocytosis. The intravesicular change of pH can be detected by a fluorescence change of a pH-sensitive derivative of green fluorescent protein (pHluorin). SynaptopHluorin (SpH) is a fusion protein of pHluorin and syntaxin (VAMP2) for targeting to secretory vesicles. Thy1-SpH transgenic mouse expresses SpH in brain under the control of neuron-specific Thy1.2 promoter. This strain is useful for studies of presynaptic terminals in various regions of the brain. Six lines of the transgenic mice with different SpH expression pattern are available from RIKEN BRC. For detailed expression pattern of SpH in each line, please see the reference listed below.

 

Depositor : Dr. Hiromu Yawo
(Graduate School of Life Sciences, Tohoku University)Dr. Yuchio Yanagawa
(Graduate School of Medicine, Gunma University)
Reference : Araki, R., Sakagami, H., Yanagawa, Y., Hikima, T., Ishizuka, T., and Yawo, H.
Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortex.
Genesis. 42:53-60, 2005