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June 2015 Genetic tool for controlled G-CaMP7 expression in vivo


Title201506

Genetic tool for controlled G-CaMP7 expression in vivo

C57BL/6J-Tg(tetO-G-CaMP7,-DsRed2)572Bsi (RBRC06510)

201506

Courtesy of Masaaki Sato, Ph.D. and Yasunori Hayashi, M.D., Ph.D.

Expression patterns of G-CaMP7 (top, green), coexpressed DsRed2 (middle, red), and G-CaMP7 overlaid with DsRed2 and Hoechst nuclear counterstaining (bottom, blue) in adult TRE-G-CaMP7 × CaMKII-tTA mice. Parasagittal sections cut at different mediolateral levels are shown from left to right.

 

Ca2+ is an intracellular messenger that regulates cellular functions such as excitability, gene expression, secretion, and contraction. Genetically encoded calcium indicators (GECIs), such as GFP-based G-CaMPs, produce great changes in fluorescence in response to increases in intracellular Ca2+ concentrations. Understanding brain function requires techniques for monitoring the patterns of activity in neuronal networks, and many transgenic mouse lines that express G-CaMPs in the brain have been reported. Hayashi and colleagues generated a new transgenic strain that expresses the improved G-CaMP variant G-CaMP7 [1] under a tetracycline response element (TRE) [2]. Crossing with tTA driver mice and doxycycline treatment results in cell type-specific and temporally controlled expression of G-CaMP7 in TRE-G-CaMP7 mice. These technical advances will provide a powerful approach for functional and pharmacological studies of neural circuit activity in vivo..

 

Depositors : Masaaki Sato, Ph.D.
Japan Science and Technology Agency (JST)
Yasunori Hayashi, M.D., Ph.D.
RIKEN Brain Science Institute (BSI)
Strain name : C57BL/6J-Tg(tetO-G-CaMP7,-DsRed2)572Bsi
RBRC No. : RBRC06510
References : [1] Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J. Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLOS ONE; 7(12):e51286, 2012.
[2] Sato M, Kawano M, Ohkura M, Gengyo-Ando K, Nakai J, Hayashi Y. Generation and imaging of transgenic mice that express G-CaMP7 under a tetracycline response element. PLOS ONE; 10(5):e0125354, 2015.

 

June 2015
Contact: Shinya Ayabe, Ph.D.
Experimental Animal Division, RIKEN BioResource Center
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