Strain Data Sheet
RBRC06016
Strain Information | |
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| Image | |
| BRC No. | RBRC06016 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | ICR-Tg(Nes-NLS-mCherry)1Imayo |
| Former Common name | Nestin-mCherry-NLS ; Nes-NLS/mCherry Tg#1 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2011. The transgene was injected into the pronuclei of ICR fertilized eggs. |
| Strain description | Nes-mCherry transgenic mice. Nuclear-localized mCherry is expressed by neural stem/progenitor cells. |
| Colony maintenance | Carrier x Noncarrier [or Crossing to Slc:ICR] |
| References | Nat. Neurosci., 18(5):657-65 (2015). doi: 10.1038/nn.3989. 25821910 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)UNnlsrat Nestin promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Ordering Information | |
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| Donor DNA | Rat Nestin promoter, Nestin 2nd intron enhancer, Nuclear-localization signal from SV40 largeT antigen, HA (hemagglutinin) tag sequence, Discosoma sp. mCherry cDNA, SV40 PolyA signal |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). |
| Depositor | Drs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |