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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

RIKEN BioResource Center Mouse E-mail Message No. 98

RIKEN BioResource Center (BRC) is pleased to update you with the latest

======= Contents =======================================================
- Special topic: Mouse of the Month
"Cre-mediated recombination in villus epithelial cells of the intestine"
- Announcement: RIKEN BRC is closed for the summer from Aug. 19 - 21
- Report: RIKEN BRC/Nanjing University MARC 2nd International Short
  Summer Course of the Mouse
- User publications
- Newly deposited mouse strains
- Newly available mouse strains
- Mouse strains for freeze preservation

|  Special topic: Mouse of the Month    July 2013

Cre-mediated recombination in villus epithelial cells of the intestine
  RBRC03801  C57BL/6N-Tg(Vil1-cre)2Utr/Rbrc
  Depositor: RIKEN BioResource Center

Mouse of the Month archive

|  Announcement: RIKEN BRC is closed for the summer from Aug. 19 - 21.

  We would like to inform you that RIKEN will be closed for business
from August 19 to 21, 2013 as a continuing policy for conserving
electricity, energy and environment. All our operations regarding
bioresources and clerical work will be suspended during this period.
  Thank you very much for your understanding.

  Period: From August 19, Monday to August 21, Wednesday, 2013
  Our operations to be suspended:
    · Telephone inquiry and email response
    · Processing of orders on bioresources
    · Shipment of bioresources
    · Sending quotations and invoices
    · All clerical work

|  Report: RIKEN BRC/Nanjing University MARC 2nd International Short
|  Summer Course of the Mouse

  Model Animal Research Center (MARC), Nanjing University and RIKEN
BioResource Center (BRC) co-organized a short educational program
focusing on mouse genetics and related experimental technologies for
young scientists. This summer, Nanjing University MARC hosted the 2nd
program from July 29 to July 31 in Nanjing, China.

|  User publications

  The following results have been newly reported by users. We are
honored to introduce new publications to the science community.
Therefore, we look forward to receiving your publications too.

RBRC00265  STM1
RBRC00266  STM2
RBRC00428  Mol-KOR5/Stm
RBRC00429  Mol-KOR7/Stm
RBRC00430  Mol-AIZ/Stm
RBRC00431  Mol-Mae/Stm
RBRC00655  KJR/Ms
RBRC00657  HMI/Ms
RBRC01164  MBT/Pas
Bamunusinghe D, Liu Q, Lu X, Oler A, Kozak CA. Endogenous
gammaretrovirus acquisition in Mus musculus subspecies carrying
functional variants of the XPR1 virus receptor. J Virol., 2013.

RBRC00209  MSM/Ms
RBRC00821  TgN(deGFP)18Imeg
RBRC00822  TgN(deGFP)20Imeg
Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M,
Greally JM, Abe K. Large, Male Germ Cell-Specific Hypomethylated DNA
Domains With Unique Genomic and Epigenomic Features on the Mouse X
Chromosome. DNA Res., 2013.

RBRC01106  semaphorin III/D (conditional) Targeting mice
Zhou J, Wen Y, She L, Sui YN, Liu L, Richards LJ, Poo MM. Axon position
within the corpus callosum determines contralateral cortical projection.
Proc Natl Acad Sci U S A.;110(29):E2714-23, doi: 10.1073/pnas.
1310233110, 2013.

RBRC01390  Nrf2 knockout mouse/C57BL6J
Nakajima Y, Nakamura Y, Shigeeda W, Tomoyasu M, Deguchi H, Tanita T,
Yamauchi K. The Role of Tumor Necrosis Factor-α and Interferon-γ in
Regulating Angiomotin-Like Protein 1 Expression in Lung Microvascular
Endothelial Cells. Allergol Int., 2013.

RBRC01834  B6-Tg(CAG-FLPe)36
Imamura T, Ueki M, Oda Y, Model Animal for Studying Hair Growth Cycle.
US20130174285 A1, 13/819,659

RBRC02498  ICR.Cg-Tg(Stra8-EGFP)1Ysa/YsaRbrc
Chihara M, Ikebuchi R, Otsuka S, Ichii O, Hashimoto Y, Suzuki A, Saga Y,
Kon Y. Mice Stage-Specific Claudin 3 Expression Regulates Progression of
Meiosis in Early Stage Spermatocytes. Biol Reprod.;89(1):3, doi: 10.1095
/biolreprod.113.107847, 2013.

RBRC03435  Arx-1 KO
Miyabayashi K, Katoh-Fukui Y, Ogawa H, Baba T, Shima Y, Sugiyama N,
Kitamura K, Morohashi K. Aristaless Related Homeobox Gene, Arx, Is
Implicated in Mouse Fetal Leydig Cell Differentiation Possibly through
Expressing in the Progenitor Cells. PLOS ONE;8(6):e68050, doi: 10.1371/
journal.pone.0068050, 2013.

|  Newly deposited mouse strains

  The following mice have been newly deposited with RIKEN BRC and will
be available shortly after rederivation.
  We appreciate the contributions of the depositors to the science

  Dr. Akihiko Yoshimura, Keio University
    RBRC04753  B6;129S4(Cg)-Socs1<tm1Ayos>
    RBRC04754  B6;129S4-Socs3<tm1Ayos>
    RBRC05756  B6.Cg-Spry2<tm1Ayos>
    RBRC05757  B6.Cg-Spry4<tm1Ayos>
    RBRC05758  B6.Cg-Spred1<tm1Hiin>
    RBRC05759  B6.Cg-Spred2<tm1Taga>
    RBRC05760  B6.Cg-Spred1<tm1.1Ayos>
    RBRC05761  B6.Cg-Rftn1<tm1Ayos>
    RBRC05762  B6.Cg-Trafd1<tm1Ayos>

  Dr. Hidenori Senpuku, National Institute of Infectious Diseases
    RBRC05946  NOD.Cg-H2<d>

  Dr. Tadatsugu Taniguchi, The University of Tokyo, Graduate School of
    RBRC05949  B6.129P2-Rbm3<tm1Ttg>

  Dr. Toru Takumi, RIKEN Brain Science Institute
    RBRC05954  B6.129S7-Dp(7Herc2-Mkrn3)1Taku

  Dr. Kuniya Abe, RIKEN BioResource Center 
    RBRC05955  B6D2-Tg(Vps52-Vps52)1Rbrc

  Dr. Masahiro Oka, Graduate School of Frontier Biosciences, Osaka
    RBRC06029  B6;129S2-Kpna4<tm1Yyo>
    RBRC06030  B6;129S2-Kpna1<tm1.1Yyo>
    RBRC06031  B6.129S2-Kpna1<tm1.2Yyo>
    RBRC06032  C57BL/6-Gt(ROSA)26Sor<tm1(KPNA1)Yyo>/75
    RBRC06033  C57BL/6-Gt(ROSA)26Sor<tm1(KPNA1)Yyo>/85
    RBRC06034  B6;129S2-Ranbp1<tm1Yyo>

|  Newly available mouse strains

  The following mice have recently been rederived into our barrier
facility and are now available for distribution.

    RBRC04023  B6C3-Senp2<Gt(venusTrap_fJAV0002-D)4004-321>
    RBRC04720  B6;129S-Rap1a<tm1Morz> Rap1b<tm1Morz>
    RBRC05517  B6;129S4-Xbp1<tm1Tiw>
    RBRC05744  B6.Cg-Tg(Grm6-cre)7Tfur
    RBRC05769  B6(129S6)-Tg(Aif1-tTA)54Kftnk
    RBRC05846  B6.Cg-Tg(Tph2-tTA)1Ahky
    RBRC05851  C.Cg-Flg<tm1>
    RBRC05854  B6.Cg-Tg(IgkAK23)
    RBRC05955  B6D2-Tg(Vps52-Vps52)1Rbrc

|  Mouse strains for freeze preservation

  Since a sufficient number of frozen stocks have been preserved, live
mice of the following strains will be discontinued soon.
  It will take more than 3 months to recover live mice from frozen stock.
Therefore, please inform us as soon as possible if you plan to use the
mice and request them alive.

    RBRC02295  B6.Cg-p130Cas<tm1Homy> Tg(BLG-cre)1Homy
    RBRC03327  TL1 KO
    RBRC03935  C57BL/6N-Tg(Insl-cre)28Utr/Rbrc
    RBRC03986  B6.129-Ep400<tm2Fuku>
    RBRC04747  STOCK Tg(aMHC-GCaMP2)60Bsi
    RBRC04938  B6.B6D2-Tg(A1AT-Spp1)25
    RBRC04948  B6.B6CB(Cg)-Psmg1<tm1.1Smta>
    RBRC05147  B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo>
    RBRC05271  p16-luc (Acc. No. CDB0416T-53)
    RBRC05363  STOCK Tg(FLEXCAG-VSFP2.3)511Bsi
    RBRC05384  Prdm14-mVenus (Acc. No. CDB0461T)
    RBRC05447  B6.Cg-Tg(ACTB-tTS)1Mllo
    RBRC05451  B6;129-Nlgn3<tm1(STOP-tetO)Rhn>
    RBRC05452  B6.Cg-Nlgn3<tm1.1(tetO)Rhn>
    RBRC05453  B6(Cg)-Tg(tetO-ChR2*C128S/EYFP)1Kftnk
    RBRC05502  129-Cfm2<tm1Tfur>
    RBRC05517  B6;129S4-Xbp1<tm1Tiw>
    RBRC05601  B6.129(Cg)-Rab7<tm1.1Ywd>
    RBRC05765  B6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko>

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RIKEN BioResource Center
Experimental Animal Division
3-1-1 Koyadai, Tsukuba-shi
Ibaraki, 305-0074 Japan