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RIKEN BioResource Center Mouse E-mail News No.124

RIKEN BioResource Center Mouse E-mail News   No. 124

RIKEN BioResource Center (BRC) is pleased to update you with the latest

======= Contents ===============================================
- Special topic: Mouse of the Month 
  "One-step generation of multiple transgenic mouse lines"
- IMPC International Symposium in Yokohama 
- User publications
- Newly deposited mouse strains

|  Special topic: Mouse of the Month    September 2015

One-step generation of multiple transgenic mouse lines
  RBRC06517 C57BL/6-Gt(ROSA)26Sor<tm10(PITT)Maoh>

Depositor: Masato Ohtsuka, Ph.D.
           Department of Molecular Life Science
           Tokai University School of Medicine

Mouse of the Month archive

|  IMPC International Symposium in Yokohama

  We are pleased to announce that IMPC International Symposium in Yokohama
will be held on Thursday, November 12th at Yokohama Port Opening Memorial Hall.
In this symposium, most important leaders of the IMPC will give introduction 
on the project. Also, related research frameworks in Japan will be reported. 
We will discuss how to establish collaborative networks not only with academia 
but with industry in order to maximize the biomedical value of mouse resources. 

Date & Time: November 12th, from 13:00 
Place: Yokohama Port Opening Memorial Hall, Yokohama, Japan
Admission fee: Free

Please register in advance on the Application Form.

|  User publications

  The following results have been newly reported by users. We are
honored to introduce new publications to the science community.
Therefore, we look forward to receiving your publications too. 

RBRC00144 C57BL/6-Ly5.1
Omi A, Enomoto Y, Kiniwa T, Miyata N, Miyajima A. Mature resting 
Ly6Chigh natural killer cells can be reactivated by IL-15. Eur J 
Immunol.;44(9):2638-47, 2014.

RBRC01345 Emx1-Cre KI Δneo
Takagi T, Nishizaki Y, Matsui F, Wakamatsu N, Higashi Y. De novo 
inbred heterozygous Zeb2/Sip1 mutant mice uniquely generated by 
germ-line conditional knockout exhibit craniofacial, callosal and 
behavioral defects associated with Mowat-Wilson syndrome. 
Hum Mol Genet., pii: ddv350, 2015.

RBRC01361 C57BL-p53+/-
Okahashi N, Kohno S, Kitajima S, Matsuda F, Takahashi C, Shimizu H. 
Metabolic characterization of cultured mammalian cells by mass 
balance analysis, tracer labeling experiments and computer-aided 
simulations. J Biosci Bioeng., doi: 10.1016/j.jbiosc.2015.04.003, 2015.

RBRC01835 C57BL/6-Tg(CAG-FLPe)37Ito/ItoRbrc [B6-Tg(CAG-FLPe)37]
Omori Y, Chaya T, Yoshida S, Irie S, Tsujii T, Furukawa T.
Identification of G Protein-Coupled Receptors (GPCRs) in Primary 
Cilia and Their Possible Involvement in Body Weight Control. 
PLoS One.;10(6):e0128422, 2015.

RBRC01835 C57BL/6-Tg(CAG-FLPe)37Ito/ItoRbrc [B6-Tg(CAG-FLPe)37]
Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, 
Sato M, Ohtsuka M. Assessment of Artificial MiRNA Architectures 
for Higher Knockdown Efficiencies without the Undesired Effects 
in Mice. PLoS One.;10(8):e0135919, 2015.

RBRC02095 B6.B6D2-Tg(Th-EGFP)21-31Koba [B6.Cg-Tg(TH-GFP)21-31]
Masi A,, Narducci R, Resta F, Carbone C, Kobayashi K, Mannaioni G. 
Differential contribution of Ih to the integration of excitatory 
synaptic inputs in Substantia Nigra pars compacta and Ventral 
Tegmental Area dopaminergic neurons. Eur J Neurosci., 
doi: 10.1111/ejn.13066, 2015.

Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, 
Abe M, Sato T, Ochiai M, Sakuraba Y, Aoyagi Y, Gondo Y, Sakimura 
K, Nakagama H, Mishima Y, Kominami R. Bcl11b SWI/SNF-complex subunit 
modulates intestinal adenoma and regeneration after γ-irradiation 
through Wnt/β-catenin pathway. Carcinogenesis;36(6):622-31, 2015.

RBRC02370 B6;129-Grn<tm1> [Progranulin knock-out mouse]
Noguchi T, Ebina K, Hirao M, Kawase R, Ohama T, Yamashita S, 
Morimoto T, Koizumi K, Kitaguchi K, Matsuoka H, Kaneshiro S, 
Yoshikawa H. Progranulin plays crucial roles in preserving bone 
mass by inhibiting TNF-α-induced osteoclastogenesis and promoting 
osteoblastic differentiation in mice. Biochem Biophys Res Commun.
;465(3):638-43, 2015.

RBRC02591 GIT2(-) (Acc. No. CDB0414K)
Yu-E Hao, Dong-Fang He, Rong-Hua Yin, Hui Chen, Jian Wang, Shao-Xia 
Wang, Yi-Qun Zhan, Chang-Hui Ge, Chang-Yan Li, Miao Yu, Xiao-Ming 
Yanga. GIT2 deficiency attenuates concanavalin A-induced hepatitis 
in mice. FEBS Open Bio.;5:688-704, 2015.

RBRC02711 B6.129S-Osmr<tm1Mtan> [Oncostatin M receptor KO mouse, OSMR-KO]
Guo S, Li ZZ, Gong J, Xiang M, Zhang P, Zhao GN, Li M, Zheng A, 
Zhu X, Lei H, Minoru T, Li H. Oncostatin M Confers Neuroprotection 
against Ischemic Stroke. J. Neurosci.;35(34):12047-62, 2015

RBRC02711 B6.129S-Osmr<tm1Mtan> [Oncostatin M receptor KO mouse, OSMR-KO]
Janssens K, Maheshwari A, Van den Haute C,  Baekelandt V, 
Stinissen P, Hendriks JJ, Slaets H, Hellings N. Oncostatin 
M protects against demyelination by inducing a protective 
microglial phenotype. Glia.;63(10):1729-37, 2015.

RBRC04109 B6.129S6-Calca<tm1Hku> [alphaCGRP-]
Xiang L, Ma L, Wei N, Wang T, Yao Q, Yang B, Xiong Y, Wu Y, Gong P. 
Effect of lentiviral vector overexpression α-calcitonin gene-related 
peptide on titanium implant osseointegration in α-CGRP-deficient mice.
Bone, doi: 10.1016/j.bone.2015.08.009, 2015.

RBRC05323 129-Sirt1<tm1Mcby> [STOCK Sirt1<tm1Mcby>]
Tomita T, Hamazaki J, Hirayama S, McBurney MW, Yashiroda H, 
Murata S. Sirt1-deficiency causes defective protein quality 
control. Sci Rep.;5:12613, 2015.

|  Newly deposited mouse strains

Dr. Masato Ohtsuka, Tokai University
RBRC06517 C57BL/6-Gt(ROSA)26Sor<tm7Maoh>

Dr. Hirokazu Inoue, Shiga University of Medical Science 
RBRC09238 C57BL/6-Tg(CAG-CCND1)14Hzi

Dr. Koichi Furukawa, Nagoya University
RBRC09317 B6CBA-B4galt1<tm1Kfk>
RBRC09318 B6.Cg-B4galt1<tm1Kfk>
RBRC09319 B6CBA-St8sia1<tm1Kfk>
RBRC09320 B6.Cg-St8sia1<tm1Kfk>
RBRC09321 B6.Cg-St3gal5<tm1Kfk>
RBRC09322 B6.Cg-A4galt<tm1Kfk>

Dr. Takashi Maruyama, Gifu University
RBRC09394 C57BL/6-Gt(ROSA)26Sor<tm1(CAG-Neo, Nfkbiz-IRES-EGFP)>

Dr. Tomoki Chiba, University of Tsukuba
RBRC09401 B6.CBA-Psme4<tm1Tchi>
RBRC09402 B6.CBA-Ecm29<tm1Tchi>
RBRC09403 B6-Klhl7f/f<tm1Tchi>
RBRC09404 B6.CBA-Park2<tm1Tchi>
RBRC09405 B6-Klhl7-/-<tm1Tchi>

Dr. Ichiro Taniuchi, RIKEN IMS
RBRC09411 B6.129.P-Zbtb7b<tm1.1Itan>

Dr. Hiroko Tsukamura, Nagoya University
RBRC09413 ICR.Cg-Tg(Kiss1-AcGFP)1Nurep
RBRC09414 ICR.Cg-Tg(Kiss1-AcGFP)2Nurep
RBRC09415 ICR.Cg-Tg(Kiss1-AcGFP)3Nurep
RBRC09416 ICR.Cg-Tg(Kiss1-AcGFP)4Nurep
RBRC09417 ICR.Cg-Tg(Kiss1-AcGFP)5Nurep

Dr. Hiromitsu Tanaka, Nagasaki International University
RBRC09390 C57BL/6-Nkapl<tm1(KOMP)Vlcg>
RBRC09408 C57BL/6-Nkapl<tm1(KOMP)Vlcg>/2Htan
RBRC09409 ICR.B6-Nkapl<tm1(KOMP)Vlcg>/1Htan
RBRC09410 ICR.B6-Nkapl<tm1(KOMP)Vlcg>/2Htan

Dr. Tetsuichiro Saito, Chiba University
RBRC09425 B6.129-Nepro<tm1.1(Venus)Tets> delta neo
RBRC09426 B6.129-Nepro<tm1(Venus)Tets> flox
RBRC09427 B6.129-Nepro<tm2(Venus)Tets> flox

Dr. Keiji Itaka, University of Tokyo
RBRC09428 B6.Cg-Adarb1<tm1.1Skwa>Tg(SLC18A3-cre)KMisa

Dr. Atsushi Iwama, Chiba University
RBRC09435 B6-Gt(ROSA)26Sor<tm1(Stop-3xFlag ESET,EGFP)>

Dr. Atsushi Miyawaki, RIKEN BSI
RBRC09447 Gt(ROSA)26Sor<em1(CAG-flox stop-SRAI)Amiy>
RBRC09448 Gt(ROSA)26Sor<em1(CAG-flox stop-mt-SRAI')Amiy>

Dr. Kazuhiro Iwai, Kyoto University
RBRC04489 B6.B6CB-Hoil1<tm1Kiwa>

Dr. Kazuaki Yoshikawa, Osaka University
RBRC09363 ICR.Cg-Ndn<tm1Ky>
RBRC09382 B6.Cg-Ndn<tm1Ky>(N20)

Dr. Yoh Wada, Osaka University
RBRC09431 Atp6v1g2<tm1.1Ywd>

Dr. Tatsuya Kusudo, Chubu University
RBRC09460 B6-Tg(HSA-FABP3)1Tkusu
RBRC09461 B6-Tg(HSA-FABP3)2Tkusu

Dr. Kazuto Kobayashi, Fukushima Medical University
RBRC09464 B6.Cg-Tg(ChAT-IL-2Rα/mVenus) 997-6

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