{"id":6432,"date":"2009-02-10T15:24:45","date_gmt":"2009-02-10T06:24:45","guid":{"rendered":"http:\/\/mus.brc.riken.jp:7301\/?page_id=6432"},"modified":"2021-04-08T10:46:16","modified_gmt":"2021-04-08T01:46:16","slug":"feb_2009_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/en\/mouse_of_month\/feb_2009_mm","title":{"rendered":"Feb 2009 The Tsix\/Xist-deficient Strains<\/em> Models for Epigenetics Study"},"content":{"rendered":"

\"mouse_of_month_200902\"<\/a><\/p>\n\n\n\n
\n

The Tsix\/Xist-deficient Strains<\/em>
\nModels for Epigenetics Study<\/h2>\n
B6.Cg-Xisttm2Sado<\/sup>\u00a0(Xist1loxGFP<\/sup>)<\/em> RBRC 01260<\/a><\/h5>\n
B6.Cg-Tsixtm1Sado<\/sup>\u00a0(TsixdeltaSA<\/sup>)<\/em> RBRC 01949<\/a><\/h5>\n
B6.Cg-Xisttm1Sado<\/sup>Tsixtm1Enl<\/sup>\u00a0(Xist1lox<\/sup>\u00a0TsixAA2delta1.7<\/sup>)<\/em> RBRC 01950<\/a><\/h5>\n
B6.Cg-Tsix\/Xisttm3Sado<\/sup>\u00a0(XistpA<\/sup>)<\/em> RBRC 02653<\/a><\/h5>\n
B6.Cg-Xisttm4Sado<\/sup>\u00a0(XistIVS19<\/sup>)<\/em> RBRC 02654<\/a><\/h5>\n
B6.Cg-Xisttm5Sado<\/sup>\u00a0(XistdeltaA<\/sup>)<\/em> RBRC 02655<\/a><\/h5>\n
Tsix\u00a0<\/em>: X (inactive)-specific transcript, antisense gene on Chr X<\/strong>
\nXist\u00a0<\/em>: inactive X specific transcripts gene on Chr X<\/strong><\/h5>\n

\"mn0902_0101\"<\/p>\n

Xist1loxGFP<\/sup><\/em>\u00a0mice RBRC 01260<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n
\"mn0902_0102\"<\/td>\n<\/td>\n\"mn0902_0103\"<\/td>\n\u00a0<\/strong><\/td>\nIn mammals, females have twice as many copies of genes on X chromosome as males do and develop a mechanism to equalize dosage of X-linked gene products by inactivating one of two X chromosomes (Lyon, 1961).
\nThe X-inactivation, also known as Lyonization, is regulated by the noncoding Xist<\/em> gene and its antisense non-coding Tsix<\/em> gene on Chr X (Sado et al<\/em>., 2001).
\nThe targeted disruption of the Xist<\/em> gene demonstrated that the expression of the Xist<\/em> gene is essential to initiate inactivating X chromosome in cis<\/em> (Marahrens et al., 1997; Penny et al., 1996).
\nThe expression of the Xist<\/em> gene was shown to be suppressed by the Tsix<\/em> gene through modifications of chromatin structure (Sado et al<\/em>., 2005).
\nThese strains provide unique and useful models to study the mechanism of X-inactivation in mammals and biological functions of noncoding RNA (Table 1<\/a>).<\/td>\n<\/tr>\n
(\u2191) Female embryos carrying mutated Xist1lox<\/sup><\/em> allele derived from the father died soon after implantation due to a dysplasia of extra-embryonic tissues. (\u2190) Normal embryos. scale bar = 200\u03bcm.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n
Depositor<\/td>\n:<\/td>\n\u00a0Dr. Takashi Sado, Division of Human Genetics, National Institute of Genetics<\/a><\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n[1]<\/td>\n\u00a0Lyon M.F. 1961. Gene action in the X-chromosome of the mouse (Mus musculus<\/em>\u00a0L.).
\nNature<\/em>\u00a0190: 372-373.<\/a><\/td>\n<\/tr>\n
[2]<\/td>\nMarahrens Y., Panning B., Dausman J., Strauss W., Jaenisch R., 1997.\u00a0Xist<\/em>-deficient mice are defective in dosage compensation but not spermatogenesis.Genes Dev 11 (2): 156-166.<\/a><\/td>\n<\/tr>\n
[3]<\/td>\nPenny G.D., Kay G.F., Sheardown S.A., Rastan S., Brockdorff N. 1996. Requirement for\u00a0Xist<\/em>in X chromosome inactivation. Nature 379 (6561): 131-137.<\/a><\/td>\n<\/tr>\n
[4]<\/td>\nSado, T., Hoki, Y., and Sasaki, H. 2005.\u00a0Tsix<\/em>\u00a0silences\u00a0Xist<\/em>\u00a0through modification of chromatin structure.\u00a0Dev Cell<\/em>\u00a09: 159-165.<\/a><\/td>\n<\/tr>\n
[5]<\/td>\nSado T., Wang Z., Sasaki H., Li E. 2001. Regulation of imprinted X-chromosome inactivation in mice by\u00a0Tsix<\/em>.\u00a0Development<\/em>\u00a0128 (8): 1275-1286.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

The Tsix\/Xist-deficient Strains Models for Epigenetics Study B6.Cg-Xisttm2Sado\u00a0(Xist1loxGFP) RBRC 01260 B6.Cg-Tsixtm1Sado\u00a0(TsixdeltaSA) RBRC 01949 B6.Cg-Xisttm1SadoTsixtm1Enl\u00a0(Xist1lox\u00a0TsixAA2delta1.7) RBRC 01950 B6.Cg-Tsix\/Xisttm3Sado\u00a0(XistpA) RBRC 02653 B6.Cg-Xisttm4Sado\u00a0(XistIVS19) RBRC 02654 B6.Cg-Xisttm5Sado\u00a0(XistdeltaA) RBRC 02655 Tsix\u00a0: X (inactive)-specific transcript, antisense gene on Chr X Xist\u00a0: inactive X specific transcripts gene on Chr X Xist1loxGFP\u00a0mice RBRC 01260   \u00a0 In mammals, females have twice as many copies of genes on X chromosome as males do and develop a mechanism to equalize dosage of X-linked gene products by inactivating one of two X chromosomes (Lyon, 1961). The X-inactivation, also known as Lyonization, is regulated by the noncoding Xist gene and its antisense non-coding Tsix gene on Chr X (Sado et al., 2001). The targeted disruption of the Xist gene demonstrated that the expression of the Xist gene is essential to initiate inactivating X chromosome in cis (Marahrens et al., 1997; Penny et al., 1996). The expression of the Xist gene was shown to be suppressed by the Tsix gene through modifications of chromatin structure (Sado et al., 2005). These strains provide unique and useful models to study the mechanism of X-inactivation in mammals and biological functions of noncoding RNA (Table 1). (\u2191) Female embryos carrying mutated Xist1lox allele derived from the father […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6432","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/6432","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=6432"}],"version-history":[{"count":12,"href":"http:\/\/mus.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/6432\/revisions"}],"predecessor-version":[{"id":18210,"href":"http:\/\/mus.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/6432\/revisions\/18210"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=6432"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}