{"id":13859,"date":"2016-08-29T13:32:40","date_gmt":"2016-08-29T04:32:40","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=13859"},"modified":"2021-04-07T13:20:13","modified_gmt":"2021-04-07T04:20:13","slug":"aug_2016_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/aug_2016_mm","title":{"rendered":"Monitoring cell replacement and migration in vivo<\/i> with Kaede"},"content":{"rendered":"

\"Title201606\"<\/a><\/p>\n\n\n\n
\n

Monitoring cell replacement and migration
in vivo<\/i> with Kaede<\/b><\/span><\/p>\n

B6.Cg-c\/c Tg(CAG-tdKaede)15Utr (RBRC05738)<\/a><\/h5>\n
B6.Cg-Tg(CAG-tdKaede)15Utr (RBRC05737)<\/a><\/h5>\n

\"201608\"<\/a><\/p>\n

Courtesy of Yoshihiro Miwa, Ph.D.<\/p>\n

Irradiation with 405 nm violet light to the lower body of Kaede mouse changed fluorescence from green to red.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\n

Kaede is a photoconvertible fluorescent protein which was cloned from a stony coral Trachyphyllia geoffroyi<\/i>. Kaede changes its color from green to red within a few minutes after exposure to violet light [1]. In transgenic mice carrying Kaede cDNA under the CAG promoter, all the cell types constitutively exhibit green fluorescence signal [2]. Immune cells in lymphoid organs and peripheral tissues in Kaede mice can be labeled in red by brief exposure to violet light [3]. Kaede mice are a useful tool to monitor cell replacement at the photoconverted site and\/or cell migration to other regions in the body.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n
Depositor<\/td>\n:<\/td>\nYoshihiro Miwa, Ph.D.
\nFaculty of Medicine
\nUniversity of Tsukuba<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nB6.Cg-c\/c Tg(CAG-tdKaede)15Utr<\/i><\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC05738<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nB6.Cg-Tg(CAG-tdKaede)15Utr<\/b><\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC05737<\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n[1]<\/td>\nAndo R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci USA<\/em>; 99(20):12651-6, 2002.<\/a><\/td>\n<\/tr>\n
[2]<\/td>\nTomura M, Yoshida N, Tanaka J, Karasawa S, Miwa Y, Miyawaki A, Kanagawa O. Monitoring cellular movement in vivo with photoconvertible fluorescence protein “Kaede” transgenic mice. Proc Natl Acad Sci USA<\/em>; 105(31):10871-6, 2008.<\/a><\/td>\n<\/tr>\n
[3]<\/td>\nTomura M, Honda T, Tanizaki H, Otsuka A, Egawa G, Tokura Y, Waldmann H, Hori S, Cyster JG, Watanabe T, Miyachi Y, Kanagawa O, Kabashima K. Activated regulatory T cells are the major T cell type emigrating from the skin during a cutaneous immune response in mice. J Clin Invest.<\/em>; 120(3):883-93, 2010.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
August 2016
\nContact: Shinya Ayabe, Ph.D.<\/a>
\nExperimental Animal Division, RIKEN BioResource Center
\nAll materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Monitoring cell replacement and migration in vivo with Kaede B6.Cg-c\/c Tg(CAG-tdKaede)15Utr (RBRC05738) B6.Cg-Tg(CAG-tdKaede)15Utr (RBRC05737) Courtesy of Yoshihiro Miwa, Ph.D. Irradiation with 405 nm […]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-13859","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/13859","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=13859"}],"version-history":[{"count":6,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/13859\/revisions"}],"predecessor-version":[{"id":18603,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/13859\/revisions\/18603"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=13859"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}