{"id":13914,"date":"2016-09-28T10:30:56","date_gmt":"2016-09-28T01:30:56","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=13914"},"modified":"2021-04-07T14:36:54","modified_gmt":"2021-04-07T05:36:54","slug":"sep_2016_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/sep_2016_mm","title":{"rendered":"A new CreERT2 line for studying PDGFR\u03b1-expressing cells in vivo<\/i>"},"content":{"rendered":"

\"Title201609\"<\/a><\/p>\n\n\n\n
\n

A new CreERT2 line for studying
PDGFR\u03b1-expressing cells in vivo<\/i><\/b><\/span><\/p>\n

B6.Cg-Pdgfra<tm1.1(EGFP\/cre\/ERT2)Hyma><\/i> (RBRC09616)<\/a><\/h5>\n

\"201609\"<\/a><\/p>\n

Courtesy of Takumi Era, M.D., Ph.D.<\/p>\n

E14.5 (top) and E20.5 (bottom) embryo of PDGFR\u03b1-GFP\/CreERT2 mice crossed with ROSA26-Tomato (left) or ROSA26-LacZ (right) reporter mice. Cre activity was induced by intraperitoneal injection of 4-hydroxytamoxifen in pregnant female mice at E9.5.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\n

Mesoderm and its descendant cells arerequired for organogenesis, wound repair, and disease pathogenesis. It can be classified into cells positive for vascular endothelial growth factor receptor 2 (VEGFR2 or FLK1) or platelet-derived growth factor receptor \u03b1 (PDGFR\u03b1), representing lateral and paraxial mesoderm, respectively. Era and colleagues generated knock-in mice carrying GFP-Cre-ERT2 at the start codon of Pdgfra<\/i> [1]. They confirmed Cre activity in mesoderm cells at gastrulation and the mesenchyme of E9.5. Neural crest cells expressing PDGFR\u03b1 was also confirmed to contribute to the facial mesenchyme. PDGFR\u03b1-GFP\/CreERT2 mouse strain is a useful tool to investigate novel role of PDGFR\u03b1-expressing cells and their descendant cells in vivo<\/i>.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n
Depositor<\/td>\n:<\/td>\nTakumi Era, M.D., Ph.D.
\nDepartment of Cell Modulation
\nInstitute of Molecular Embryology and Genetics, Kumamoto University<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nB6.Cg-Pdgfra<tm1.1(EGFP\/cre\/ERT2)Hyma><\/i><\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC09616<\/td>\n<\/tr>\n
Reference<\/td>\n:<\/td>\n[1]<\/td>\nMiwa H, Era T. Generation and characterization of PDGFR\u03b1-GFPCreERT2 knock-In mouse line. Genesis<\/em>; 53(5):329-36, 2015.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
September 2016
\nContact: Shinya Ayabe, Ph.D.<\/a>
\nExperimental Animal Division, RIKEN BioResource Center
\nAll materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

A new CreERT2 line for studying PDGFR\u03b1-expressing cells in vivo B6.Cg-Pdgfra<tm1.1(EGFP\/cre\/ERT2)Hyma> (RBRC09616) Courtesy of Takumi Era, M.D., Ph.D. E14.5 (top) and E20.5 (bottom) embryo of PD […]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-13914","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/13914","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=13914"}],"version-history":[{"count":7,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/13914\/revisions"}],"predecessor-version":[{"id":18604,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/13914\/revisions\/18604"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=13914"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}