{"id":15092,"date":"2018-11-30T09:26:39","date_gmt":"2018-11-30T00:26:39","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=15092"},"modified":"2022-07-13T15:10:15","modified_gmt":"2022-07-13T06:10:15","slug":"dec_2018_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/dec_2018_mm","title":{"rendered":"Bicistronic Insulin1-Cre knock-in mice"},"content":{"rendered":"\n\n\n
\"RIKEN<\/td>\n December 2018<\/span>
\nMouse of the Month<\/span><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
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Bicistronic Insulin1-Cre knock-in mice<\/b><\/span><\/p>\n

C57BL\/6J-Ins1<em1(cre)Utr> (RBRC09525)<\/a><\/h5>\n

\"FigAB_201812\"<\/a><\/p>\n

Reproduced with permission of Japanese Association for Laboratory Animal Science from Yoshikazu Hasegawa, et al.<\/i>, 2016. Generation of CRISPR\/Cas9-mediated bicistronic knock-in ins1-cre driver mice. Exp. Anim.<\/i> 65(3): 319\u2013327.<\/p>\n

(A) Strategy of bicistronic cre expression in I2AC mice
\nThe Cre recombinase expression cassette was integrated before stop codon of Insulin1<\/i> gene by the CRISPR\/Cas9 system.
\n(B) The adult pancreas of (R26GRR \u00d7 C57BL\/6J-Ins1<\/i><em1(cre)Utr>) F1 mice.
\nR26GRR mice (RBRC04874) is a Cre-reporter mouse strain, which shows EGFP signals in non-Cre-recombined cells and tdsRed signals in Cre-recombined [1]. The tdsRed-positive islet (by Cre\/loxP recombination) is co-localization with insulin-positive islet (by immunofluorescent analysis).\n<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\n

Diabetes mellitus is a metabolic disorder due to impaired insulin action. Diabetic pathogenic mechanism is related to genetic factors. Therefore, in vivo<\/i> experiments with genetically modified mice is important. Especially, the control of gene expression specifically in pancreas is effective.<\/p>\n

I2AC (I<\/u>nsulin1-2A<\/u>–C<\/u>re recombinase) KI mice is a novel Cre-driver strain [2]. This strain enables the conditional gene expression in the pancreas by Cre\/loxP system. The character of I2AC mice is mainly two as compared to conventional Insulin Cre-driver mice. One is that Cre recombinase expression is regulated by endogenous Insulin1<\/i> promoter, not Insulin2<\/i>. This fact allows the restricted Cre recombinase expression only in pancreatic beta cells. The other is that 2A self-cleaving peptide is inserted prior to Cre recombinase gene. This fact allows the bicistronic expression without interruption of endogenous Insulin1 expression. Actually, I2AC mice showed no significant differences in results of glucose tolerance tests. Thus, I2AC mice will provide a new insight into glucose metabolic study.\n<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n
Depositor<\/td>\n:<\/td>\nFumihiro Sugiyama, Ph.D.
\nLaboratory Animal Resource Center
\nUniversity of Tsukuba<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nC57BL\/6J-Ins1<em1(cre)Utr><\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC09525<\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n[1]<\/td>\nHasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Mizuno-Iijima S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K. Novel ROSA26 Cre-reporter knock-in C57BL\/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. Exp Anim.<\/em>; 62(4): 295-304, 2013.<\/a><\/td>\n<\/tr>\n
[2]<\/td>\nHasegawa Y, Hoshino Y, Ibrahim AE, Kato K, Daitoku Y, Tanimoto Y, Ikeda Y, Oishi H, Takahashi S, Yoshiki A, Yagami K, Iseki H, Mizuno S, Sugiyama F. Generation of CRISPR\/Cas9-mediated bicistronic knock-in ins1-cre driver mice. Exp Anim.<\/em>; 65(3): 319-327, 2016.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
December 2018
\nContact: Saori Mizuno, Ph.D.<\/a>
\nExperimental Animal Division, RIKEN BioResource Research Center
\nAll materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

December 2018 Mouse of the Month   Bicistronic Insulin1-Cre knock-in mice C57BL\/6J-Ins1<em1(cre)Utr> (RBRC09525) Reproduced with permission of Japanese Association for Laboratory Animal Sci […]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-15092","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/15092","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=15092"}],"version-history":[{"count":6,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/15092\/revisions"}],"predecessor-version":[{"id":19144,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/15092\/revisions\/19144"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=15092"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}