{"id":15219,"date":"2019-01-30T15:42:06","date_gmt":"2019-01-30T06:42:06","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=15219"},"modified":"2021-04-07T13:46:06","modified_gmt":"2021-04-07T04:46:06","slug":"feb_2019_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/feb_2019_mm","title":{"rendered":"iRFP transgenic mice for in vivo<\/i> imaging"},"content":{"rendered":"

\"title201902\"<\/a><\/p>\n\n\n\n
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iRFP transgenic mice for in vivo<\/i> imaging<\/b><\/span><\/p>\n

B6.Cg-c\/c Tg(CAG-iRFP)#846Utr (RBRC10067)<\/a><\/h5>\n
B6.Cg-c\/c Tg(CAG-iRFP)#867Utr (RBRC10068)<\/a><\/h5>\n

\n\"Fig_201902\"<\/a>\n<\/p>\n

Courtesy of Yoshihiro Miwa, Ph.D.<\/p>\n

Transplantation of cells from iRFP Tg mouse (left) visualizes accumulation of the cells non-invasively in recipient (right).<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
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Near-infrared (NIR) fluorescent protein (iRFP) is a nontoxic fluorescent protein that can be excited at 690 nm and emits fluorescence at 713 nm (1). Because iRFP is brighter, stronger and more stable than previous fluorescent proteins, these advantages allow the great deep tissue penetration and minimal autofluorescence in vivo<\/i>; that is, ubiquitous iRFP transgenic mice (RBRC10067 and RBRC10068) are useful for in vivo<\/i> imaging (2). Actually, depositor (Dr. Miwa) and his colleague established in vivo<\/i> murine atherosclerosis imaging system by using iRFP transgenic mice (3). They reported that this novel imaging approach predictably enable to monitor lesion progression without an invasive method or injection of imaging reagents.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n
Depositor<\/td>\n:<\/td>\nYoshihiro Miwa, Ph.D.
\nDepartment of Molecular Pharmacology (Fluorescence Bioimaging)
\nFaculty of Medicine, University of Tsukuba<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nB6.Cg-c\/c Tg(CAG-iRFP)#846Utr<\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC10067<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nB6.Cg-c\/c Tg(CAG-iRFP)#867Utr<\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC10068<\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n[1]<\/td>\nFilonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV., Bright and stable near-infrared fluorescent protein for in vivo imaging. Nat Biotechnol.<\/em>; 29(8): 757-61, 2011.<\/a><\/tr>\n
[2]<\/td>\nTran MT, Tanaka J, Hamada M, Sugiyama Y, Sakaguchi S, Nakamura M, Takahashi S, Miwa Y. In vivo image analysis using iRFP transgenic mice. Exp Anim.<\/em>; 63(3): 311-9, 2014.<\/a><\/td>\n<\/tr>\n
[3]<\/td>\nKulathunga K, Hamada M, Hiraishi Y, Otake M, Tran MTN, Cheng O, Tanaka J, Sakasai T, Sakaguchi S, Sugiyama Y, Fleischmann BK, Takahashi S, Miwa Y. A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System. Sci Rep.; 8(1): 14515, 2018.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
February 2019
\nContact: Saori Mizuno, Ph.D.<\/a>
\nExperimental Animal Division, RIKEN BioResource Research Center
\nAll materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

iRFP transgenic mice for in vivo imaging B6.Cg-c\/c Tg(CAG-iRFP)#846Utr (RBRC10067) B6.Cg-c\/c Tg(CAG-iRFP)#867Utr (RBRC10068) Courtesy of Yoshihiro Miwa, Ph.D. Transplantation of cells from iRFP Tg mou […]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-15219","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/15219","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=15219"}],"version-history":[{"count":20,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/15219\/revisions"}],"predecessor-version":[{"id":18634,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/15219\/revisions\/18634"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=15219"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}